紫皮石斛总酚提取工艺优化及其抗氧化活性研究  

Optimization of Extraction Process and Antioxidant Activity of Total Phenol in Dendrobium devoninum

作　　者：董寿堂[1] 杨斐然 任远[1] 张旭强[1] 王怀斌 鲁静[1] 郭艳东 何云龙 杨红芹[1] Dong Shoutang;Yang Feiran;Ren Yuan;Zhang Xuqiang;Wang Huaibin;Lu Jing;Guo Yandong;He Yunlong;Yang Hongqin(Baoshan College of Traditional Chinese Medicine,Baoshan 678000,China;Baoshan People's Hospital,Baoshan 678000,China)

机构地区：[1]保山中医药高等专科学校,云南保山678000 [2]保山市人民医院,云南保山678000

出　　处：《中国野生植物资源》2023年第6期53-58,共6页Chinese Wild Plant Resources

基　　金：云南省教育厅治疗痹证中医中药工程研究中心(培育)(云教发[2022]70号);云南省教育厅科学研究基金项目(2023J1809);保山中医药高等专科学校科技创新团队项目(2020t001)。

摘　　要：目的:研究紫皮石斛总酚提取工艺及体外抗氧化活性。方法:在单因素试验的基础上,采用响应面法优化紫皮石斛总酚的超声波辅助提取工艺条件,通过测定紫皮石斛总酚对超氧阴离子、DPPH自由基及羟自由基的清除率,评价其抗氧化活性。结果:紫皮石斛总酚最优提取工艺条件为乙醇浓度69%,料液比1∶40(g/mL),提取时间31 min,在此条件下总酚提取量为1.56 mg/g,与理论值1.58 mg/g接近。紫皮石斛总酚对超氧阴离子、羟自由基及DPPH自由基的半数抑制浓度(IC50)分别为19.40μg/mL、118.35μg/mL及30.75μg/mL。结论:优选的紫皮石斛总酚提取工艺合理可行,且其具有较强的体外抗氧化活性。Objective:To study the extraction process and antioxidant activity in vitro of total phenol from Dendrobium devoninum.Methods:On the basis of single factor experiments,the ultrasonic‑assisted extraction of total phenol from D.devoninum was optimized by response surface methodology.The antioxidant activity of total phenol from D.devoninum was evaluated by measuring the scavenging rates of superoxide anion,DPPH radical and hydroxyl radical.Results:The optimal extraction conditions were as follow:ethanol concentration of 69%,solid-liquid ratio of 1∶40(mg/mL)and extraction time of 31 min.Under these conditions,the extraction content of total phenol was 1.56 mg/g,which was close to the predicted value of 1.58 mg/g.The half‑maximal inhibitory concentration(IC50)of the extracted total phenol from D.devoninum on hydroxyl radical,superoxide anion radical and DPPH radical was 19.40μg/mL,118.35μg/mL and 30.75μg/mL,respectively.Conclusion:The optimized extraction technology of total phenol from D.devoninum is reasonable and feasible,and it has had certain antioxidant activities in vitro.

关 键 词：紫皮石斛 总酚 响应面法 超声辅助提取 抗氧化 

分 类 号：R284.2[医药卫生—中药学]