甘蓝型油菜千粒重全基因组关联分析  

Genome wide association analysis of thousand-seed weight in Brassica napus L.

作　　者：肖小军[1,2] 韩德鹏 周会汶 肖国滨[2] 郑伟[2] 李亚贞[2] 黄天宝[2] 肖富良 陈明[2] 吕伟生 周庆红[1] XIAO Xiao-jun;HAN De-peng;ZHOU Hui-wen;XIAO Guo-bin;ZHENG Wei;LI Ya-zhen;HUANG Tian-bao;XIAO Fu-liang;CHEN Ming;LYU Wei-sheng;ZHOU Qing-hong(College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China;Jiangxi Institute of Red Soil/Jiangxi Key Laboratory of Red Soil Arable Land Conservation/National Engineering and Technology Research Cen-ter for Red Soil Improvement,Nanchang 330046,China;Jiujiang University,Jiujiang 332005,China)

机构地区：[1]江西农业大学农学院,江西南昌330045 [2]江西省红壤研究所/江西省红壤耕地保育重点实验室/国家红壤改良工程技术研究中心,江西进贤330046 [3]九江学院,江西九江332005

出　　处：《中国油料作物学报》2023年第3期510-517,共8页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31860392,32160463);江西省重点研发计划(20203BBF63034);国家油菜产业技术体系建设专项(CARS-12);江西省油菜产业技术体系专项(JXARS-08)。

摘　　要：为指导千粒重遗传改良,挖掘甘蓝型油菜千粒重显著关联单核苷酸多态性(SNP)位点及相关候选基因,以300份甘蓝型油菜种质资源为材料,对千粒重进行一年两地表型考察,结合该群体前期开发的201 817个SNP标记,采用一般线性模型(GLM)和混合线性模型(MLM)进行全基因组关联分析,对性状显著关联SNP位点两侧100kb区域内相关候选基因进行功能预测。结果表明,300份甘蓝型油菜千粒重在两地均表现出广泛的表型变异,筛选出6份千粒重较高的油菜种质资源。基于GLM、MLM模型分别检测到24个和10个与油菜千粒重显著关联的SNP,其中有8个SNP在2个模型中均被检测到,位于第C02号染色体上Bn-C02-22853028的表型贡献率最大。6个位点附近找到HWS、DA1、DA2、CPL3、bZIP、CSPs、PTR2、ARF2、TRM61等9个拟南芥已报道粒重基因的同源基因。To better understand the genetics of thousand seed weight(TSW)of rapeseed,SNP loci and candidate genes were studied using 300 Brassica napus resources.Data were investigated under 2 environments(Nanchang and Jinxian)in the same year,and genome-wide association study(GWAS)was carried out using 201817 SNPs markers.Results showed that the extensive phenotypic variations existed between in the 2 sites,and 6 materials with higher TSWs were identified.24 and 10 SNPs were respectively excavated by general linear model and mixed linear model in the 2 sites.Among them,8 SNPs were detected in both models.The phenotypic contribution of Bn-C02-22853028 on chromosome C02 was the largest.9 candidate homologous genes documenting Arabidopsis seed weight were found near the 6 SNP loci,including HWS,DA1,DA2,CPL3,bZIP,CSPs,PTR2,ARF2 and TRM61.The above results were ex-pected to facilitate understanding the genetic basis of TSW and rapeseed breeding.

关 键 词：甘蓝型油菜 全基因组关联分析 千粒重 SNP位点 同源基因 

分 类 号：S565.4[农业科学—作物学]