凡纳滨对虾二氧化碳麻醉后有水保活运输条件优化  

Optimization of keep-alive transportation conditions with water after carbon dioxide anesthesia for Litopenaeus vannamei

作　　者：盛祥 刘文畅[1,2] 谭洪新 罗国芝[1,2] 孙大川 朱博文[3] 庞云 扶泽发[3] SHENG Xiang;LIU Wenchang;TAN Hongxin;LUO Guozhi;SUN Dachuan;ZHU Bowen;PANG Yun;FU Zefa(Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China;Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-culture of Aquaculture Animals,Shanghai 201306,China;Innovative Recirculating Aquaculture Systems(Nanjing)Co.,Ltd.,Nanjing 210019,China)

机构地区：[1]上海海洋大学上海水产养殖工程技术研究中心,上海201306 [2]上海市水产动物良种创制与绿色养殖协同创新中心,上海201306 [3]智循环(南京)水产科技有限责任公司,南京210019

出　　处：《上海海洋大学学报》2023年第3期532-543,共12页Journal of Shanghai Ocean University

基　　金：上海市科学技术委员会扬帆专项(22YF1416400);上海水产养殖工程技术研究中心能力提升项目(19DZ2284300)。

摘　　要：为提高凡纳滨对虾(Litopenaeus vannamei)在二氧化碳(Carbon dioxide,CO_(2))麻醉后有水运输过程中的存活率,研究CO_(2)流速(麻醉阶段)、浸泡时间(浸泡阶段)、水体类型和盐度(复苏阶段)对复苏效果的影响。通过设置CO_(2)流速(3、5和7 L/min)、浸泡时间(0、1和5 min)、水体类型(自来水、养殖水上清液和养殖水)以及盐度(3、7和11)等4个因素,进行单因素试验,并以结果为依据进行L_(9)(3^(4))正交和验证试验。单因素试验结果表明,最佳CO_(2)流速为7 L/min、最佳浸泡时间为0 min、最佳水体类型为自来水、最佳盐度为7;正交试验结果显示,当复苏时间为2 h时,4个因素对运输效果影响的主次关系为浸泡时间>水体类型>盐度>CO_(2)流速,最佳运输方式组合为CO_(2)流速为8 L/min、水体类型为自来水、盐度为7、浸泡时间为0 min;当复苏时间为4 h时,4个因素对运输效果影响的主次关系为浸泡时间>CO_(2)流速>水体类型>盐度,最佳运输方式组合为CO_(2)流速为7 L/min、水体类型为自来水、盐度为7、浸泡时间为0 min;当复苏时间为6 h时,4个因素对运输效果影响的主次关系为浸泡时间>CO_(2)流速>盐度>水体类型,最佳运输方式组合为CO_(2)流速为6 L/min、水体类型为自来水、盐度为7、浸泡时间为0 min。研究结果表明,可通过调节CO_(2)流速、浸泡时间、水体类型和盐度等4个因素的水平,提高凡纳滨对虾在不同运输时长条件下的复苏率,为对虾保活运输提供了新的思路。In order to improve the survival rate of Litopenaeus vannamei during transportation with water after carbon dioxide(CO_(2))anesthesia,the effects of CO_(2)flow rates(anesthesia stage),soaking time(soaking stage),water type and salinity(recovery stage)on the survival rate were studied.The experiment set four factors:CO_(2)flow rates(3 L/min,5 L/min and 7 L/min),soaking time(O min,1 min and 5 min),water type(tap water,supernatant of aquaculture water and aquaculture water)and salinity(3,7 and 11).The single factor experiment was carried out,and the L_(9)(3^(4))orthogonal experiment and verification experiment were carried out based on the single factor experimental results.The results showed that in the single factor experiment,the best CO_(2)flow rates was 7 L/min,the best soaking time was O min,the best water type was tap water and the best salinity was 7.The results of orthogonal experiment of transportation within 2 hours showed that the primary and secondary relationship of the four factors was:soaking time>water type>salinity>CO_(2)flow rates,and the best combination was CO_(2)flow rates of 8 L/min,the water type of tap water,the salinity of 7 and the soaking time of O min.The results of orthogonal experiment of transportation within 4 hours showed that the primary and secondary relationship of the four factors was soaking time>CO_(2) flow rates>water type>salinity,and the best combination was CO_(2)flow rates of 7 L/min,the water type of tap water,the salinity of 7 and the soaking time of O min.The results of orthogonal experiment of transportation within 6 hours showed that the primary and secondary relationship of the four factors was soaking time>CO_(2)flow rates>salinity>water type,and the best combination was CO_(2)flow rates of6 L/min,the water type of tap water,the salinity of 7 and the soaking time of O min.The experimental results show that the recovery rate of Litopenaeus vannamei under different transportation time conditions can be improved by adjusting the levels of four factors:CO_(2)flow rates

关 键 词：凡纳滨对虾 CO_(2) 保活运输 条件优化 

分 类 号：S981.21[农业科学—捕捞与储运]