Combined antibacterial and antibiofilm activity of phenyllactic acid and bacteriocin XJS01 against Shigella flexneri  

作　　者：Yu-Hang Jiang Lin-Yu Yang Wei-Gang Xin Qi-Lin Zhang 

机构地区：[1]Faculty of Life Science and Technology,Kunming University of Science and Technology,Yunnan Kunming,650500,China

出　　处：《Food Bioscience》2022年第1期266-273,共8页食品生物科学（英文）

基　　金：supported by Yunnan Fundamental Research Projects(Grant No.202101BE070001-046);the Natural Science Foundation of China(31960286).

摘　　要：This study investigated antibacterial and antibiofilm activity of the combined use of phenyllactic acid(PLA)and bacteriocin XJS01 against Shigella flexneri_14.The minimum inhibitory concentration(MIC)of PLA and XJS01 against S.flexneri_14 was 2.45 mg/mL and 18.75μg/mL,respectively.Growth and kill kinetics assays showed that the combined use of 1/2MIC PLA plus 1/2MIC XJS01 had a better activity against planktonic S.flexneri_14 compared to treatment with PLA and XJS01 used singly(1/2MIC and 2MIC).Cellular biochemical and morphological analysis revealed the remarkable ability of the combination in disrupting cell appearance and promoting deformation of planktonic S.flexneri_14 compared to single use.Moreover,S.flexneri_14 biofilm formation was inhibited and degraded by the combination,which showed a more remarkable antibiofilm activity than PLA and XJS01 when used singly.This study demonstrates the synergistic antibacterial activity of PLA and XJS01 against S.flexneri_14 in either planktonic or biofilm states in foods.

关 键 词：Foodborne pathogenic bacteria Shigella flexneri BACTERIOCIN Phenyllactic acid Synergistic antibacterial activity Antibiofilm activity 

分 类 号：TS201[轻工技术与工程—食品科学]