Extraction of protein from churpi of yak milk origin:Size reduction,nutraceutical potential and as a wall material for resveratrol  

作　　者：Asir Gani Nairah Noor Adil Gani Jenno Joseph-Leenose-Helen Asima Shah Zanoor ul Ashraf 

机构地区：[1]Laboratory of Functional Food and Nutraceuticals,Department of Food Science and Technology,University of Kashmir,Srinagar,190006,India

出　　处：《Food Bioscience》2022年第2期900-911,共12页食品生物科学（英文）

基　　金：Dr Adil Gani is thankful to National Mission on Himalayan studies(MoEF&CC).Ref.No:GBPNI/NMHS-2017-18/SG-19/618/326/138;Department of Biotechnology,Ref.No.DBT-RA/2021/January/N/860;Department of Science&Technology(INSPIRE),Ref no No:DST/INSPIRE Fellowship/[IF180189]for their financial assistance.

摘　　要：Resveratrol is a known anti-oxidant and anti-cancer bioactive.The low bioavailability and poor water solubility of resveratrol is a major barrier that restricts its usage in food applications.To overcome these problems,the study presents micro and nano encapsulation of resveratrol in casein micelles extracted from Himalayan cheese(Churpi).Resveratrol loaded casein microparticles(CS-rm)were obtained using emulsion-freeze drying.Whereas,resveratrol loaded casein nanoparticles(CS-rn)were obtained using ultrasonication.Both were characterized using dynamic light scattering(DLS),scanning electron microscopy(SEM)and attenuated total reflectance-fourier transform infrared spectroscopy(ATR-FTIR).DLS revealed hydrodynamic diameter of 71.24μm and 387.023 nm and zeta potential of-22.62 and-28.08 mV for CS-rm and CS-rn.FTIR confirmed the characteristic peaks of resveratrol at 965.4,1380.86,1586.66 and 1607 cm-1 confirmed successful micro and nanoencapsulation.CS-rm displayed high encapsulation efficiency(52.32%)and swelling power(77.90 at pH 3 and 68.65 at pH 7.5)than CS-rn.Both micro and nanoencapsulation protected resveratrol in gastric conditions,however,CS-rn showed high release of resveratrol then CS-rm in simulated intestinal conditions.The anti-oxidant and anti-cancer activity of resveratrol under simulated gastric and intestinal conditions(SGID)was significantly higher(p≤0.05)for CS-rn,showing that nanoencapsulation improved release and nutraceutical profile of resveratrol.Resveratrol loaded nano and micro casein particles can be used a source of fortified protein supplement in growing food and nutraceutical industry.

关 键 词：CASEIN Encapsulation efficiency In vitro release ANTI-CANCER 

分 类 号：TS252.5[轻工技术与工程—农产品加工及贮藏工程]