超高效液相色谱法检测特殊医学用途配方食品中22种游离氨基酸  

Determination of 22 Free Amino Acids in Food for Special Medical Purposes by Ultra-performance Liquid Chromatography

作　　者：尹丽丽 郑红 程志 卢兰香 薛霞 王骏 胡梅 刘艳明 YIN Lili;ZHENG Hong;CHENG Zhi;LU Lanxiang;XUE Xia;WANG Jun;HU Mei;LIU Yanming(Shandong Institute for Food and Drug Control,Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes,Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug,Jinan 250101,China)

机构地区：[1]山东省食品药品检验研究院,山东省特殊医学用途配方食品质量控制工程技术研究中心,山东省食品药品安全检测工程技术研究中心,山东济南250101

出　　处：《食品工业科技》2023年第14期264-271,共8页Science and Technology of Food Industry

基　　金：2019年度山东省重点研发计划(医用食品)(2019YYSP020)。

摘　　要：本文建立了特殊医学用途配方食品中22种游离氨基酸的超高效液相色谱检测方法。样品中氨基酸用磺基水杨酸提取,以2,4二硝基氟苯(DNFB)为柱前衍生试剂,以乙腈水-甲酸铵为流动相梯度洗脱,HSS T3色谱柱高效分离,超高效液相-二极管阵列检测器检测,外标法定量。结果显示,22种氨基酸在2~100μg/mL范围内线性关系良好(R2>0.99)。方法检出限(S/N≥3)为10 mg/100 g,定量限(S/N≥10)为25 mg/100 g,低、中、高三个加标水平的回收率在90.1%~109.1%,相对标准偏差为0.8%~6.9%。方法简单高效、准确度高、重复性好,适用于特殊医学用途配方食品中22种游离氨基酸的定性、定量分析,为保障特殊医学用途配方食品的质量安全提供技术支持。A method was established to determine 22 free amino acids in food for special medical purposes by ultra-performance liquid chromatography.After extraction with sulfosalicylic acid and derivation with 2,4-dinitrofluorobenzene(DNFB),the samples were eluted with acetonitrile water-ammonium formate as mobile phase.Amino acids were separated by HSS T3 column and detected with diode-array detector by external standard method.Results showed that,the 22 amino acids were linear in the range of 2~100μg/mL with correlation coefficients higher than 0.99.The limits of detection(S/N≥3)were 10 mg/100 g,and the limits of quantification(S/N≥10)were 25 mg/100 g.The recoveries were in the range of 90.1%and 109.1%with relative standard deviation(RSD)from 0.8%to 6.9%.This method was simple,efficient,accurate and reproducible.It was suitable for determination of 22 free amino acids in food for special medical purposes,which could provide technical support to ensure the quality and safety.

关 键 词：特殊医学用途配方食品 游离氨基酸 柱前衍生 2 4二硝基氟苯 超高效液相色谱法 

分 类 号：S513[农业科学—作物学]