高原鼠兔肠道菌群移植对高原环境下SD大鼠肺动脉压、体重及采食量的影响  

Effect of Fecal Microbiota Transplantation on Hypoxic Pulmonary Hypertension,Weight Gain,and Food Intake in SD Rats at High Altitude

作　　者：陈征 王书祥 柴沙驼[1] 格日立[2] 李胜利[3] 刘书杰[1] CHEN Zheng;WANG Shu-xiang;CHAI Sha-tuo;GE Ri-li;LI Sheng-li;LIU Shu-jie(Academy of Animal and Veterinary Sciences,Qinghai University,Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province,Xining 810016,China;Research Center for High Altitude Medicine,Qinghai University,Xining 810001,China;China Agricultural University,Beijing 100193,China)

机构地区：[1]青海大学畜牧兽医科学院,青海省高原放牧家畜营养与饲料科学重点实验室,青海西宁810016 [2]青海大学高原医学中心,青海西宁810001 [3]中国农业大学动物科学技术学院,北京100193

出　　处：《青海畜牧兽医杂志》2023年第1期35-40,共6页Chinese Qinghai Journal of Animal and Veterinary Sciences

摘　　要：本试验旨在通过高原鼠兔(Ochotona curzoniae)肠道菌群移植(Fecal microbiota transplantation,FMT)方式改善高原低压、低氧导致的大鼠肠道菌群失调,并探索菌群移植对大鼠肺动脉高压及其生长性能的影响。将生活在海拔3200 m的高原鼠兔肠道菌群移植到Sprague-Dawley(SD)大鼠体内,然后转移SD大鼠至低压氧舱(模拟6000 m海拔)中饲养30 d,再进行肺动脉压肠道菌群等指标检测。结果显示FMT可显著降低SD大鼠的平均肺动脉压(mPAP),提高SD大鼠的日增重,并且降低大鼠的日采食量。16S rRNA测序结果表明,移植组大鼠肠道菌群变化显著,特别是毛螺菌科(Lachnospiraceae)和脱硫弧菌科(Desulfovibrionaceae)。综上所述,高原鼠兔对SD大鼠的菌群移植有效地改变了大鼠的肠道菌群组成,降低了肺动脉压,且改善了大鼠的生长性能。At high altitudes,low air pressure and oxygen levels can negatively impact the gut microbiome,leading to systemic inflammation and increased vulnerability to disease.To explore the effects of fecal microbiota transplantation(FMT)on pulmonary hypertension and growth and development,we transplanted gut microbes from Ochotona Curzoniae,a small mammal that lives in the Tibetan plateau at 3200m,into Sprague-Dawley(SD)rats.We then placed the rats in a hypobaric chamber at an altitude of 6000m for 30 days and measured various parameters.Our findings revealed that FMT significantly decreased mean pulmonary artery pressure(mPAP),increased daily weight gain,and reduced feed intake in SD rats.Furthermore,the 16S rRNA analysis showed notable changes in the gut microbiota of the transplanted rats,particularly in the Lachnospiraceae and Desulfovibrionaceae families.This study demonstrates the efficacy of gut microbiome manipulation via FMT in reducing pulmonary artery pressure and improving growth and development in rats.Moreover,this approach has promising implications for treating pulmonary arterial hypertension(PAH)and enhancing adaptability to high altitudes in livestock breeding.

关 键 词：菌群移植 低氧 肺动脉高压 生长性能 菌群多样性 

分 类 号：R364[医药卫生—病理学]