Remodeling of the ryanodine receptor isoform 1 channel regulates the sweet and umami taste perception of Rattus norvegicus  

作　　者：Wenli Wang Dingqiang Lu Qiuda Xu Yulian Jin Guangchang Pang Yuan Liu 

机构地区：[1]Department of Food Science&Technology,School of Agriculture&Biology,Shanghai Jiao Tong University,Shanghai 200240,China [2]College of Biotechnology&Food Science,Tianjin University of Commerce,Tianjin 300134,China

出　　处：《Fundamental Research》2023年第3期459-468,共10页自然科学基础研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(31972198,31622042,31901813,31671857,31901782).

摘　　要：Sweet and umami tastes are elicited by sweet and umami receptors on the tongue and palate epithelium,respectively.However,the molecular machinery allowing the taste reaction remains incompletely understood.Through a phosphoproteomic approach,we identified the key proteins that trigger taste mechanisms based on phosphorylation cascades.Ryanodine receptor isoform 1(RYR1)was further verified by sensory and behavioral assays.We propose a model of RYR1-mediated sweet/umami signaling in which the RYR1 channel,which mediates Ca^(2+)release from the endoplasmic reticulum,is closed by dephosphorylation in bud tissue after sweet/umami treatment.The alteration in Ca^(2+)content in the cytosol induces transient membrane depolarization and generates a cell current for taste signal transduction.We demonstrate that RYR1 is a new channel involved in the regulation of sweet/umami signal transduction and propose a“metabolic clock”notion based on sweet/umami sensing.Our study provides a valuable foundation for a system-level understanding of the taste perception mechanism.

关 键 词：UMAMI SWEET Phosphoproteomic Ryanodine receptor isoform 1 Metabolic clock 

分 类 号：TS20[轻工技术与工程—食品科学]