Lomatogonium rotatum extract alleviates diabetes mellitus induced by a high-fat,high-sugar diet and streptozotocin in rats  

作　　者：Li-Li Dai Sung-Bo Cho Hui-Fang Li Li-Sha A Xiao-Ping Ji Sirigunqiqige Pan Ming-Lan Bao Laxinamujila Bai Gen-Na Ba Ming-Hai Fu 

机构地区：[1]NMPA Key Laboratory of Quality Control of Traditional Chinese Medicine(Mongolian Medicine),Inner Mongolia Minzu University,Tongliao 028000,Inner Mongolia Autonomous Region,China [2]Key Laboratory of Tropical Translational Medicine of Ministry of Education,Hainan Provincial Key Laboratory for Research and Development of Tropical Herbs,Hainan Medical University,Haikou 571199,Hainan Province,China [3]Key Laboratory of Tropical Translational Medicine of Ministry of Education,Hainan Provincial Key Laboratory for Research and Development of Tropical Herbs,School of Pharmacy,Hainan Medical University,Haikou 571199,Hainan Province,China

出　　处：《World Journal of Diabetes》2023年第6期846-861,共16页世界糖尿病杂志（英文版）（电子版）

基　　金：Supported by the National Natural Science Foundation of China,No.82260773 and 81803845;Central Government Guided Local Scientific and Technological Development Project,No.2021ZY0015;Science and Technology Young Talents Development Project of Inner Mongolia Autonomous Region,No.NJYT22048.

摘　　要：BACKGROUND Lomatogonium rotatum(LR)is traditionally used in Mongolian folk medicine as a hypoglycemic agent,but its evidence-based pharmacological effects and mechanisms of action have not been fully elucidated.AIM To emphasize the hypoglycemic action mechanism of LR in a type 2 diabetic rat model and examine potential biomarkers to obtain mechanistic understanding regarding serum metabolite modifications.METHODS A high-fat,high-sugar diet and streptozotocin injection-induced type 2 diabetic rat model was established.The chemical composition of the LR was identified by high performance liquid chromatography.LR extract administrated as oral gavage at 0.5 g/kg,2.5 g/kg,and 5 g/kg for 4 wk.Anti-diabetic effects of LR extract were evaluated based on histopathological examination as well as the measurement of blood glucose,insulin,glucagon-like peptide 1(GLP-1),and lipid levels.Serum metabolites were analyzed using an untargeted metabolomics approach.RESULTS According to a chemical analysis,swertiamarin,sweroside,hesperetin,coumarin,1.7-dihydroxy-3,8-dimethoxyl xanthone,and 1-hydroxy-2,3,5 trimethoxanone are the principal active ingredients in LR.An anti-diabetic experiment revealed that the LR treatment significantly increased plasma insulin and GLP-1 levels while effectively lowering blood glucose,total cholesterol,triglycerides,low-density lipoprotein cholesterol,and oral glucose tolerance test compared to the model group.Furthermore,untargeted metabolomic analysis of serum samples detected 236 metabolites,among which 86 were differentially expressed between the model and the LR group.It was also found that LR considerably altered the levels of metabolites such as vitamin B6,mevalonate-5P,Dproline,L-lysine,and taurine,which are involved in the regulation of the vitamin B6 metabolic pathway,selenium amino acid metabolic pathway,pyrimidine metabolic pathway,and arginine and proline metabolic pathways.CONCLUSION These findings indicated that LR may have a hypoglycemic impact and that its role may be related to changes

关 键 词：Mongolian medicine Lomatogonium rotatum Type 2 diabetes Metabolomics SWERTIAMARIN STREPTOZOTOCIN 

分 类 号：R285.5[医药卫生—中药学]