宁夏不同子产区霞多丽葡萄内源激素及果实品质差异研究  

Effect of different terroir conditions on endogenous hormones and berry quality of Chardonnay at the East Helan Mountains

作　　者：王宁[1] 张艳霞 李栋梅 王振平[1] WANG Ning;ZHANG Yanxia;LI Dongmei;WANG Zhenping(School of Agricultural,Ningxia University,Yinchuan 750021,Ningxia,China;School of Life Science,Ningxia University,Yinchuan 750021,Ningxia,China)

机构地区：[1]宁夏大学农学院,银川750021 [2]宁夏大学生命科学学院,银川750021

出　　处：《果树学报》2023年第7期1374-1385,共12页Journal of Fruit Science

基　　金：国家自然科学基金(U20A201113);财政部和农业农村部:国家现代农业产业技术体系(CARS-29-zp-3)。

摘　　要：【目的】揭示贺兰山东麓不同子产区霞多丽葡萄果实发育过程中内源激素含量差异,了解不同产区果实品质形成的变化规律。【方法】以镇北堡产区(ZBB,砾石土,志辉源石酒庄)、玉泉营产区(YQY,风沙土,玉泉国际酒庄)、鸽子山产区(GZS,淡灰钙土,西鸽酒庄)3个子产区霞多丽葡萄为试材,使用超液相色谱-质谱联用仪(ultra performance liquid chromatography tandem mass spectrometry,UPLC-MS)测定葡萄果实发育过程中果皮、果肉和种子中脱落酸(abscisic acid,ABA)、生长素(indole acetic acid,IAA)、赤霉素(gibberellic acid,GA3)、异戊烯腺嘌呤(isopentene adenine,2-IP)和反式玉米素核苷(trans zeatin riboside,tZR)含量,使用高效液相色谱法(high performance liquid chromatography,HPLC)测定葡萄果实中葡萄糖、果糖、苹果酸、酒石酸含量。采用实时荧光定量PCR法(quantitative real-time PCR,qRT-PCR)检测脱落酸代谢途径相关基因表达量。【结果】在霞多丽葡萄果实生长发育过程中,各组织ABA含量在E-L35时期达到峰值,GZS最高;GA3总体呈“M”形变化趋势;IAA含量总体呈下降趋势,E-L 35之后种子中IAA在较低水平浮动;果皮中2-IP含量逐渐上升后在采收期下降。3个子产区霞多丽葡萄果实各组织内源激素含量存在差异,在转色期差异显著。在不同风土特征条件下,果实百粒质量和可滴定酸含量均为ZBB最高;果实单糖(果糖、葡萄糖)含量和固酸比表现为YQY>GZS>ZBB;苹果酸含量为ZBB>GZS>YQY;酒石酸含量则YQY最高,GZS最低。E-L 35时期VvNCED在GZS果实中表达量显著高于其他两个产区,ZBB霞多丽葡萄果实中VvHYD2表达量显著高于GZS和YQY。【结论】YQY种植的霞多丽葡萄含糖量和固酸比高于其他产区,表明霞多丽葡萄对YQY的风土有更好的适应性。ZBB砾石土含氮量高,有机质丰富,霞多丽葡萄果实中IAA、tZR含量高于其他产区,ABA含量低于其他产区,果实含糖量低,酸度高,成熟【Objective】This study aimed to investigate the changes of endogenous hormone content and fruit quality of Chardonnay grape cultivated in different terroir conditions,and to clarify the relationship between the endogenous hormones and fruit quality in order to provide a theoretical basis for Chardonnay flavor formation in each sub-production region of the Eastern foothills of the Helan Mountain in Ningxia.【Methods】We used Chardonnay(Vitis vinifera L.)in three sub-production areas of ZhenBeiBu(Gravelly soil,ZBB,Yuanshi vineyard),YuQuanYing(Aeolian soils,YQY,Chateau yuquan)and GeZiShan(Sierozem,GZS,Xige estate)as experimental materials.The berries were sampled at five periods,E-L 33(Berries still hard and green),E-L 35(Berries begin to colour and enlarge),E-L 37(Berries not quite ripe)and E-L 38(Berries harvest-ripe),and were frozen with liquid nitrogen.The berry skin,pulp and seed were ground to powder in liquid nitrogen and 10 mL of 80%methanol was added to 1 g of the powder.The solution was extracted overnight in the dark at 4℃in a shaker.The mixture was then centrifuged at 12000×g and 4℃for 5 min and the supernatant was collected.The extraction process was repeated three times and the collected supernatant was pooled and the methanol was evaporated off by nitrogen blowing apparatus.The remaining material was extracted by adding 1.5 times of petroleum ether,and the ether phase was discarded.The same volume of methyl for mate was added for extraction and the ester phase was collected.The ester phase obtained was blown dry by nitrogen blowing apparatus,dissolved with chromatographic methanol,passed through a 0.22μm filter membrane and stored at-20℃.The endogenous hormone content of abscisic acid(ABA),indole acetic acid(IAA),gibberellin A3(GA3),isopentene adenine(2-IP),and trans zeatin riboside(tZR)in fruit skin,flesh and seed during fruit development were determined by UPLC-MS(Ultra Performance Liquid Chromatography Tandem Mass Spectrometry).The contents of glucose,fructose,malic acid and tartaric a

关 键 词：霞多丽葡萄 产区 内源激素 果实品质 

分 类 号：S663.1[农业科学—果树学]