超声辅助有机酸预处理玉米芯制取生物乙醇的工艺优化  

Optimization of Production Technology of Ethanol from Corncob by Ultrasonic-assisted Organic Acid Pretreatment

作　　者：于德涵[1] 朱颖怡 黎莉[1] 武海玲 YU Dehan;ZHU Yingyi;LI Li;WU Hailing(Food and Pharmaceutical Engineering Department,Suihua University,Suihua,Heilongjiang 152061,China)

机构地区：[1]绥化学院食品与制药工程学院,黑龙江绥化152061

出　　处：《贵州农业科学》2023年第7期103-111,共9页Guizhou Agricultural Sciences

基　　金：黑龙江省大学生创新创业训练计划项目“玉米穗轴制备燃料乙醇的预处理和酶解工艺研究”(202110236017);黑龙江省教育厅基本科研业务费项目“玉米提取物的药用生物活性及MCM-41载药体系的研究”(YWK10236210231)。

摘　　要：【目的】探明超声辅助有机酸预处理玉米芯的最佳工艺,为玉米芯高效制取生物乙醇提供参考。【方法】通过单因素试验(柠檬酸浓度、液料比、超声功率、超声温度和超声处理时间)与响应面试验,优化有机酸预处理玉米芯工艺,以酶解效率和乙醇产率为评价指标确定最佳酶用量和发酵方式。【结果】使用浓度为5%的柠檬酸与玉米芯粉末以液料比20∶1(mL/g)混合均匀,于75℃、360 W功率条件下超声处理33 min,玉米芯还原糖得率可达36.71%;此条件下,添加纤维素酶30 FPU/g,采用半同步法发酵72 h,玉米芯乙醇产率可达70.32%;采用分步发酵和同步发酵的最高乙醇产率分别为70.08%和70.19%。【结论】超声辅助有机酸预处理玉米芯效果好,可行性高,选择不同的发酵方式时乙醇产率无明显差异。【Objective】The optimum technology of ultrasonic-assisted organic acid pretreatment of corncob was explored to to provide reference for the production of bioethanol from corncob with high efficiency.【Method】The optimal pretreatment process of corncob by organic acid was optimized by single factor experiment(citric acid concentration,material-liquid ratio,ultrasonic power,ultrasonic temperature and ultrasonic treatment time)and response surface experiment.The optimal enzyme dosage and fermentation method were determined by taking the efficiency of enzymatic hydrolysis and the yield of ethanol as evaluation index.【Result】The yield of reducing sugar was 36.71%when corncob was treated by citric acid with 5%concentration and liquid-material ratio with 20∶1 mL/g at 75℃and 360 W power for 33 min;Under this condition,30 FPU/g cellulose was added,and the ethanol yield could reach 70.32%by semi-simultaneous fermentation for 72 h;The highest ethanol yield of stepwise fermentation and simultaneous fermentation were 70.08%and 70.19%,respectively.【Conclusion】The ultrasonic-assisted organic acid pretreatment had good effect on corncob and high feasibility,and there was no significant difference in ethanol yield when different fermentation methods were selected.

关 键 词：玉米芯 有机酸 预处理 超声辅助 酶解发酵 工艺优化 乙醇产率 

分 类 号：TQ914.3[化学工程] TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]