18份凤凰单丛茶树株系遗传多样性的ISSR分析  

ISSR-based Analysis on Diversity of 18'Fenghuang Dancong'Tea Plant Strains

作　　者：刘颖瑶 黄婕妤 汪艺豪 晏嫦妤[1] LIU Ying-yao;HUANG Jie-yu;WANG Yi-hao;YAN Chang-yu(College of Horticulture,South China Agricultural University,Guangzhou,Guangdong 510642,China)

机构地区：[1]华南农业大学园艺学院,广东广州510642

出　　处：《茶叶学报》2023年第3期49-55,共7页Acta Tea Sinica

基　　金：以农产品为单元的广东省现代农业产业体系创新团队建设项目(茶叶)(2022KJ120)。

摘　　要：【目的】对凤凰单丛株系进行遗传多样性的ISSR分析,可为凤凰单丛茶树种质资源的品种选育及利用和保存提供一定理论依据。【方法】采用ISSR分子标记技术对18份凤凰单丛茶树株系进行遗传多样性分析、聚类分析和种质鉴定。【结果】6条ISSR引物共得到扩增位点40个,其中多态性条带为38条,多态性位点率达93.33%;平均多态性基因位点6.33个;平均Nei基因多样性指数(h)为0.3106,平均香农信息指数(I)为0.4754;遗传相似性系数位于0.40~0.90之间,遗传距离位于0.11~0.92。聚类分析结果为,当遗传相似系数GS为0.64时,18个样本可被分为3个类群。6个引物共扩增了3个特异标记。【结论】本研究认为,18份凤凰单丛株系具有丰富的遗传多样性。【Objective】ISSR markers were used to analyze the genetic diversity of'Fenghuang Dancong'[Camellia sinensis(L.)O.Kuntze]for breeding,utilizing,and conserving the valuable natural resource.【Method】Genetic diversity of 18 Fenghuang Dancong tea plant strains were determined using the molecular marker technology.【Result】Of the 40 sites amplified from 6 ISSR primers,38 bands were polymorphic at a rate of 93.33%.They had an average gene locus of 6.33,a Nei gene diversity index(h)of 0.3106,and a Shannon information index(I)of 0.4754.The genetic similarity coefficients of the cultivars were between 0.40 and 0.90 with the genetic distances between 0.11 and 0.92.The 18 strains were clustered into 3 groups at the genetic similarity coefficient of 0.64.The 6 ISSR primers totally amplified 3 specific markers.【Conclusion】The 18 Fenghuang Dancong tea plant strains were shown to be genetically rich in diversity.

关 键 词：茶树 凤凰单丛 ISSR 遗传多样性 

分 类 号：S571.1[农业科学—茶叶生产加工]