Complete biosynthesis of the phenylethanoid glycoside verbascoside  

作　　者：Yihan Yang Daoyi Xi Yanan Wu Tao Liu 

机构地区：[1]Key Laboratory of Engineering Biology for Low-Carbon Manufacturing,Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China [2]National Center of Technology Innovation for Synthetic Biology,Tianjin 300308,China [3]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Plant Communications》2023年第4期286-300,共15页植物通讯（英文）

基　　金：supported by the National Key Research and Development Program(2019YFA0905703);the National Natural Science Foundation of China(31970065,U1902214);the Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIPKJGG-002);the Key Research and Development Plan of Guangdong Province(2022B1111070005).

摘　　要：Verbascoside,which was first discovered in 1963,is a well-known phenylethanoid glycoside(PhG)that exhibits antioxidant,anti-inflammatory,antimicrobial,and neuroprotective activities and contributes to the therapeutic effects of many medicinal plants.However,the biosynthetic pathway of verbascoside remains to be fully elucidated.Here,we report the identification of two missing enzymes in the verbascoside biosynthesis pathway by transcriptome mining and in vitro enzymatic assays.Specifically,a BAHD acyltransferase(hydroxycinnamoyl-CoA:salidroside hydroxycinnamoyltransferase[SHCT])was shown to catalyze the regioselective acylation of salidroside to form osmanthuside A,and a CYP98 hydroxylase(osmanthuside B 3,30-hydroxylase[OBH])was shown to catalyze meta-hydroxylations of the p-coumaroyl and tyrosol moieties of osmanthuside B to complete the biosynthesis of verbascoside.Because SHCTs and OBHs are found in many Lamiales species that produce verbascoside,this pathway may be general.The findings from the study provide novel insights into the formation of caffeoyl and hydroxytyrosol moieties in natural product biosynthetic pathways.In addition,with the newly acquired enzymes,we achieved heterologous production of osmanthuside B,verbascoside,and ligupurpuroside B in Escherichia coli;this work lays a foundation for sustainable production of verbascoside and other PhGs in micro-organisms.

关 键 词：ACYLTRANSFERASE cytochrome P450 microbial synthesis VERBASCOSIDE ACTEOSIDE PHENYLPROPANOID 

分 类 号：O62[理学—有机化学]