超声-微波辅助提取酸浆宿萼多糖及其体外降糖活性  

Ultrasonic-Microwave Assisted Extraction of Polysaccharide from Calyx of Physali alkekengi L.and Its Hypoglycemic Activity

作　　者：李成华[1] 秦汝兰[1] 关颖丽[1] 付艳艳[2] 薛长松[1] 朱芳娆 陈建宇 LI Chenghua;QIN Rulan;GUAN Yingli;FU Yanyan;XUE Changsong;ZHU Fangrao;CHEN Jianyu(Laboratory for the Development and Utilization of Superior Crude Drug Resources in Changbai Mountain,Tonghua Normal University,Tonghua 134001,Jilin,China;Department of Nephrology,Affiliated Hospital of Changchun University of Chinese Medicine,Changchun 130021,Jilin,China)

机构地区：[1]通化师范学院长白山优势生药资源开发与利用实验室,吉林通化134001 [2]长春中医药大学附属医院肾病科,吉林长春130021

出　　处：《食品研究与开发》2023年第14期93-98,共6页Food Research and Development

基　　金：吉林省教育厅科学技术研究规划项目(JJKH20210532KJ);吉林省科技发展计划项目(20200404031YY);吉林省教育科学“十三五”规划项目(GH20298)。

摘　　要：该文以酸浆宿萼为试材,研究料液比、超声功率、微波功率、协同时间对酸浆宿萼多糖提取量的影响,在单因素试验的基础上,采用四因素三水平的响应面法优选提取工艺参数,通过对α-葡萄糖苷酶、α-淀粉酶抑制能力测定检测酸浆宿萼多糖体外降糖活性。结果表明:最佳提取工艺为料液比1∶17(g/mL)、超声功率160 W、微波功率320 W、协同时间4 min;在该条件下,酸浆宿萼多糖提取量为(26.13±0.11)mg/g;体外降糖试验表明,酸浆宿萼多糖有降糖活性。Taking Physali alkekengi L.as the raw material,the effects of extraction conditions such as solid-to-liquid,ultrasonic power,microwave power,time were investigated by one-factor and the response surface method,and hypoglycemic activity analyzed with inhibitory activity of α-glucosidase and α-amylase.The results shows that the optimum conditions for flavonoids extraction were determined as follows:solid-to-liquid:1∶17 (g/mL),ultrasonic power 160 W,microwave power 320 W,time 4 min;under these conditions,the extraction yield of polysaccharides from the calyx of Physali alkekengi L.was (26.13±0.11) mg/g;Polysaccharides of Physali alkekengi L.has hypoglycemic activity.

关 键 词：酸浆宿萼 多糖 超声-微波协同提取 工艺优化 降糖活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]