产环糊精葡萄糖基转移酶苦橙内生细菌的分离鉴定  

Isolation and Identification of Endophytic Bacteria Producing Cyclodextrin Glucosyltransferase from Bitter Orange

作　　者：朱国威 李丽芬 傅楠楠 李子院[1] 柴寿海 李海云[1] ZHU Guowei;LI Lifen;FU Nannan;LI Ziyuan;CHAI Shouhai;LI Haiyun(College of Chemistry&Bioengineering,Guilin University of Technology,Guilin 541004,Guangxi,China;Guangxi Guilin Pengyu Brothers Citrus Industry Development Co.,Ltd.,Guilin 542500,Guangxi,China)

机构地区：[1]桂林理工大学化学与生物工程学院,广西桂林541004 [2]广西桂林鹏宇兄弟柑桔产业开发有限责任公司,广西桂林542500

出　　处：《食品研究与开发》2023年第14期198-204,共7页Food Research and Development

基　　金：广西科技基地和人才专项(桂科AD18050004);广西高校中青年教师科研基础能力提升项目(2021KY0273)。

摘　　要：为寻找产环糊精葡萄糖基转移酶(cyclodextrin glycosyltransferase,CGTase)的微生物新菌株,采用酚酞-甲基橙筛选平板、淀粉-碘筛选平板从苦橙内生细菌中分离获得一株可产环糊精葡萄糖基转移酶的新菌株,命名为KCEB04。形态学特征、生理生化反应、16S rDNA序列同源性分析结果表明,菌株KCEB04归属于产酸克雷伯氏菌(Klebsiella oxytoca)。酶活测定结果表明,该菌株所产淀粉水解酶酶活为5 884.35 U/mL、α-CGTase酶活为1.65 U/mL、β-CGTase酶活为0.44 U/mL、γ-CGTase酶活为1.05 U/mL。To search for new microbial strains producing cyclodextrin glucosyltransferase (CGTase),a strain capable of producing CGTase was isolated from endophytic bacteria of bitter orange using phenolphthalein-methyl orange screening plates and starch-iodine screening plates,and named KCEB04.Morphological characteristics,physiological and biochemical reactions,and 16S rDNA sequence homology analysis results showed that strain KCEB04 belonged to Klebsiella oxytoca.Enzyme activity assay results showed that the strain produced 5 884.35 U/mL amylase,1.65 U/mL α-CGTase,0.44 U/mL β-CGTase,and 1.05 U/mL γ-CGTase.

关 键 词：环糊精葡萄糖基转移酶 苦橙 内生细菌 产酸克雷伯氏菌 分离鉴定 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]