1株伏马毒素B_(1)降解真菌的筛选及降解特性分析  

Screening and Degradation Characteristics of a Fumonisin B_(1)-Degrading Fungal Strain

作　　者：白青云 高红侠 胡俊强 于阳光 邱涵 张宇航 徐剑宏[2,3] BAI Qingyun;GAO Hongxia;HU Junqiang;YU Yangguang;QIU Han;ZHANG Yuhang;XU Jianhong(School of Life Science and Food Engineering,Huaiyin Institute of Technology,Huai’an 223003,China;Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base,Ministry of Science and Technology,Key Laboratory for Agro-product Safety Risk Evaluation(Nanjing),Ministry of Agriculture and Rural Affairs,Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality,Ministry of Agriculture and Rural Affairs,Collaborative Innovation Center for Modern Grain Circulation and Safety,Institute of Food Safety and Nutrition,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Key Open Laboratory of Agricultural Environment Microbiology Engineering,Ministry of Agriculture and Rural Affairs,College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]淮阴工学院生命科学与食品工程学院,江苏淮安223003 [2]江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地,农业农村部农产品质量安全控制技术与标准重点实验室,农业农村部农产品质量安全风险评估实验室(南京),江苏省现代粮食流通与安全协同创新中心,江苏省农业科学院农产品质量安全与营养研究所,江苏南京210014 [3]南京农业大学生命科学学院,农业农村部农业环境微生物工程重点开放实验室,江苏南京210095

出　　处：《食品科学》2023年第14期79-85,共7页Food Science

基　　金：国家自然科学基金面上项目(31872914);国家农产品安全风险评估项目(GJFP20220105);江苏省农业科技创新基金项目(CX(21)1005)。

摘　　要：为筛选能够降解伏马毒素B_(1)(fumonisin B_(1),FB_(1))的真菌,将伏马毒素污染严重的玉米样品磨碎处理,采用富集培养的筛菌方法获得目的菌株,将所得菌株命名为FDS-2,对其进行菌种鉴定、生长特性与降解特性分析,并探究该菌株对FB_(1)的初步降解途径。根据FDS-2的培养特征、显微特征以及rRNA-ITS序列特征构建的系统进化树,该菌被鉴定为棘状外瓶霉(Exophiala spinifera)。菌株的最适生长与降解条件为30℃、pH 5.0,在此条件下,菌株可在48h内将250μg的FB_(1)降解完全。通过降解物质定位实验,确定FDS-2中降解活性物质是位于细胞内的降解酶。使用液相色谱-质谱分析降解产物,确定初步降解途径为FDS-2首先把FB_(1)降解成为水解FB_(1)。本研究为粮食和饲料中伏马毒素的生物降解提供了菌种资源和理论基础。A fungal strain capable of degrading fumonisin B_(1)(FB_(1))from ground maize samples heavily contaminated with fumonisin was obtained by enrichment culture method.The strain was named as FDS-2,and was characterized for its growth and degradation characteristics,and the initial degradation pathway of FB_(1) by FDS-2 was investigated.The strain was identified as Exophiala spinifera according to its culture characteristics,microscopic characteristics and phylogenetic tree based on RNA-internal transcribed spacer(ITS)sequence characteristics.The optimum growth and degradation conditions were 30℃and pH 5.0.Under these conditions,the strain could completely degrade 250μg of FB_(1) within 48 h.Intracellular FB_(1)-degrading enzymes were discovered in this strain.Using liquid chromatography-mass spectrometry(LC-MS)to analyze the degradation products,it was determined that the primary degradation pathway began with the degradation of FB_(1) into hydrolyzed FB_(1).This study provides a strain resource and a theoretical basis for the biodegradation of fumonisin in grain and feed.

关 键 词：伏马毒素B_(1) 生物降解 棘状外瓶霉 降解途径 

分 类 号：TS201.3[轻工技术与工程—食品科学]