联合UPLC-QTOF-MS与HS-SPME-GC-MS测定赣南脐橙果肉化学成分  

Identification of Chemical Components in Gannan Navel Orange Pulp by UPLC-QTOF-MS Coupled with HS-SPME-GC-MS

作　　者：刘渝辰 余迎利[1,2] 甘思逸 刘宇鑫 金汝生 蔡新送 叶咏虹 毛雪金[1,2] 王远兴 LIU Yuchen;YU Yingli;GAN Siyi;LIU Yuxin;JIN Rusheng;CAI Xinsong;YE Yonghong;MAO Xuejin;WANG Yuanxing(State Key Laboratory of Food Science and Resources,Nanchang University,Nanchang 330047,China;College of Food Science,Technology Nanchang University,Nanchang 330047,China)

机构地区：[1]南昌大学食品科学与资源挖掘全国重点实验室,江西南昌330047 [2]南昌大学食品学院,江西南昌330047

出　　处：《食品科学》2023年第14期216-228,共13页Food Science

基　　金：国家自然科学基金地区科学基金项目(32160562);南昌大学食品科学与技术国家重点实验室自由探索项目(SKLF-ZZB-202129)。

摘　　要：采用超高效液相色谱-四极杆飞行时间串联质谱技术,在37 min内鉴定脐橙果肉中的148种非挥发性有机物,分别为黄酮类、有机酸类、氨基酸类和其他植物化学成分。采用顶空固相微萃取结合气相色谱-质谱联用技术,研究赣南地区4个主产区瑞金、信丰、安远和南康脐橙果肉中挥发性有机物,总共鉴定挥发性有机化合物97种,包括酯类、醇类、醛类和萜烯类化合物。研究结果为进一步分析赣南地区的脐橙果肉物质提供详细数据,对进一步研究赣南脐橙物质基础具有一定指导意义。Using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS),we separated and identified 148 non-volatile organic compounds including flavonoids,organic acids and amino acids in navel orange pulp in 37 min.The volatile organic compounds(VOCs)in the pulp of navel oranges from four major production regions in southern Jiangxi Province,Ruijin,Xinfeng,Anyuan and Nankang were detected by headspace solid phase microextraction combined with gas chromatography-mass spectrometry(HS-SPME-GC-MS).In total,97 VOCs were identified including esters,alcohols,aldehydes and terpenes.The results of this study provide detailed data for further research on chemical substances in the pulp of Gannan navel oranges.

关 键 词：超高效液相色谱-四极杆飞行时间串联质谱技术 顶空固相微萃取结合气相色谱-质谱联用 赣南脐橙 挥发性有机物 非挥发性有机物 

分 类 号：TS201.2[轻工技术与工程—食品科学]