储藏温度对石竹笋内生细菌多样性的影响  

Effect of Storage Temperature on the Diversity of Endophytic Bacteria in Phyllostachys nuda Shoots

作　　者：黄志远 毛传亮 万众 张舒涵 吕衡 毛闻君 赵西凯 冷利松 卞方圆 HUANG Zhi-yuan;MAO Chuan-liang;WAN Zhong;ZHANG Shu-han;LV Heng;MAO Wen-jun;ZHAO Xi-kai;LENG Li-song;BIAN Fang-yuan(China National Bamboo Research Center,Hangzhou 310012,Zhejiang,China;Qingyuan Natural Resources and Planning Bureau of Zhejiang,Qingyuan 323800,Zhejiang,China;Anji Forestry Bureau,Anji 313300,Zhejiang,China;Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;The People′s Government of Hanggai Town,Anji 313305,Zhejiang,China)

机构地区：[1]国家林业和草原局竹子研究开发中心,浙江杭州310012 [2]庆元县自然资源和规划局,浙江庆元323800 [3]安吉县林业局,浙江安吉313300 [4]浙江农林大学,浙江杭州311300 [5]杭垓镇人民政府,浙江安吉313305

出　　处：《竹子学报》2023年第2期48-55,共8页Journal of Bamboo Research

基　　金：浙江省省院合作林业科技项目(2020SY01)。

摘　　要：【目的】探究储藏温度对石竹(Phyllostachys nuda)笋采后内生细菌多样性的影响,为石竹笋采后的储藏保鲜技术进一步发展提供一定理论依据。【方法】以石竹笋为研究对象,利用高通量测序技术对其在不同储藏温度下(-20℃、4℃、25℃)的内生细菌进行测序并进行生物信息学分析。【结果】在经过不同温度储藏后,各处理间石竹笋发生了不同程度腐败作用。与-20℃储藏相比,4℃和25℃储藏分别发生了较轻和较重的腐败作用。此外,石竹笋采后在不同储藏温度下,内生细菌的Alpha多样性发生显著变化,其特征为随着储藏温度的增加呈现上升趋势;Beta多样性亦发生显著变化,表明不同储藏温度对石竹笋内生细菌的群落结构有显著改变。石竹笋内生细菌群落组成变化方面,在门水平上,变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、蓝藻门(Cyanobacteria)和放线菌门(Actinobacteria)等4个门微生物变化最为显著,其中变形菌门为最优势门。与-20℃相比,25℃和4℃储藏都显著增加了厚壁菌门的相对丰度,显著减少了变形菌门的相对丰度;25℃显著增加了放线菌门的相对丰度,显著减少了蓝藻门的相对丰度,同时4℃显著增加了蓝藻门的相对丰度。在属水平上,沙雷氏菌属(Serratia)、假单胞菌属(Pseudomonas)、欧文氏菌属(Erwinia)、乳球菌属(Lactococcus)和Lelliottia等5个属的细菌物种相对丰度有较为明显变化。与-20℃相比,25℃和4℃都显著增加了乳球菌属和Lelliottia属物种的相对丰度,同时4℃储藏也增加了沙雷氏菌属和欧文氏菌属物种的相对丰度,而25℃储藏增加了假单胞菌属物种的相对丰度。【结论】总体而言,储藏温度的降低会显著降低放线菌门、厚壁菌门微生物的相对丰度,同时也显著降低假单胞菌属微生物的相对丰度,这些门属中的微生物通常具有促腐败作用。因此,储藏温度降低可以有效抑制并【Objective】To investigate the effect of storage temperature on the diversity of endophytic bacteria of the harvested Phyllostachys nuda shoots so as to provide a theoretical basis for the further development of storage and preservation technology for the post-harvested Ph.nuda shoots.【Method】This paper takes Ph.nuda shoots as the object of study,the endophytic bacteria of Ph.nuda shoots were sequenced at different storage temperatures(-20℃,4℃and 25℃)and the bioinformatics were analyzed by using high-throughput sequencing technology.【Result】The results showed that Ph.nuda shoots underwent different degrees of spoilage between various treatments after storage at different temperatures.Compared with storage at-20℃,less and more severe spoilage occurred at 4℃and 25℃,respectively.In addition,the Alpha diversity of endophytic bacteria of the shoots changed significantly at different storage temperatures after harvesting,being characterized with an upward trend as the storage temperature increased;the Beta diversity also changed significantly,indicating that different storage temperatures significantly changed the community structure of endophytic bacteria in Ph.nuda shoots.With regard to the changes in the composition of endophytic bacteria communities in Ph.nuda shoots,at the phylum level,four phyla,Proteobacteria,Firmicutes,Cyanobacteria and Actinobacteria,showed the most significant changes in the composition of the endophytic community,with Proteobacteria being the most dominant phyla.Compared with storage at-20℃,storage at both 25℃and 4℃significantly increased the relative abundance of Firmicutes and significantly decreased the relative abundance of Proteobacteria;storage at 25℃significantly increased the relative abundance of Actinobacteria and significantly decreased the relative abundance of Cyanobacteria,while 4℃significantly increased the relative abundance of Cyanobacteria.At the genus level,the relative abundance of microorganisms in five genera,i.e.Serratia,Pseudomonas,Erw

关 键 词：石竹笋 储藏温度 内生细菌 微生物多样性 高通量测序 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]