Characterization of the chemical fungicides-responsive and bacterial pathogen-preventing Bacillus licheniformis in rice spikelet  

作　　者：Chengfang Zhan Mengchen Wu Hongda Fang Xiaoyu Liu Jiuyue Pan Xiaoyan Fan Mengcen Wang Haruna Matsumoto 

机构地区：[1]State Key Laboratory of Rice Biology&Ministry of Agricultural and Rural Affairs Laboratory of Molecular Biology of Crop Pathogens and Insects,Zhejiang University,Hangzhou,China [2]Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province,Institute of Pesticide and Environmental Toxicology,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou,China [3]Zhejiang Laboratory for Systems&Precision Medicine,Zhejiang University Medical Center,Hangzhou,China [4]School of Medicine,Zhejiang University,Hangzhou,China [5]College of Plant Protection,Hunan Agricultural University,Hunan,China [6]Plant Genetics,TUM School of Life Sciences,Technical University of Munich,Freising,Germany [7]Global Education Program for AgriScience Frontiers,Graduate School of Agriculture,Hokkaido University,Sapporo,Japan

出　　处：《Food Quality and Safety》2023年第2期256-266,共11页食品品质与安全研究（英文版）

基　　金：supported by the National Key R&D Program of China(No.2021YFE0113700);the National Natural Science Foundation of China(No.32122074);the Fundamental Research Funds for the Central Universities(No.2021FZZX001-31);the Programme for High-Level Talents Cultivation of Zhejiang University,and the Strategic Research on‘Plant Microbiome and Agroecosystem Health'(No.2020ZL008,Cao Guangbiao High Science and Technology Foundationof Zhejiang University),China。

摘　　要：Seed-borne bacterial pathogens cause severe yield loss and biotoxin contamination in rice,leading to increasing concern on the global food supply and environmental safety.Plant native microbes play an important role in defending against diseases,but their actions are often influ-enced by the chemical fungicides applied in the field.Here,Bacillus licheniformis mmj was isolated from rice spikelet,which uniquely showed not only fungicide-responsiveness but also broad-spectrum antimicrobial activity against major rice bacterial pathogens including Xanthomonas oryzae pv.oryzae,Burkholderia plantari and Burkholderia glumae.To understand the hallmark underlying the environmental adaptation and anti-microbial activity of B.licheniformis mmj,the genome sequence was determined by SMRT and subjected to bioinformatics analysis.Genome sequence analysis enabled the identification of a set of antimicrobial-resistance and antibacterial activity genes together with an array of harsh environment-adaptive genes.Moreover,B.licheniformis mmj metabolites were analyzed with gas chromatography coupled to triple quadrupole mass spectrometry,and the volatile components that were linked with the antimicrobial activity were preliminarily profiled.Collectively,the present findings reveal the genomic and metabolic landscapes underlying fungicide-responsive B.licheniformis,which offers a new opportunity to design harsh environment-adaptive biopesticides to cope with prevalent bacterial phytopathogens.

关 键 词：Bacillus licheniformis fungicide adaptation antimicrobial activity GENOMICS METABOLITES rice production 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]