Malondialdehyde treatment reduced immunoreactivity of amandin and delayed its digestion  

作　　者：Xin Luo Mingyan Ai Yongyan Wu Qia Wang Hongbo Song Qun Huang Jiankang Lu 

机构地区：[1]Production&Construction Group Key Laboratory of Special Agricultural Products Further Processing in Southern Xinjiang,Tarim University,Ala'er,China [2]College of Food Science,Fujian Agriculture and Forestry University,Fuzhou,China [3]School of Public Health,The Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,Guiyang,China [4]Wuhan Academy of Agricultural Sciences,Wuhan,China [5]Institute for Egg Science and Technology,School of Food and Biological Engineering,Chengdu University,Chengdu,China [6]Key Laboratory of Endemic and Ethnic Diseases,Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou Province,Guizhou Medical University,Guiyang,China

出　　处：《Food Quality and Safety》2023年第2期277-290,共14页食品品质与安全研究（英文版）

基　　金：supported by from the National Natural Science Foundation of China(No.31860431).

摘　　要：Objectives:The secondary products of lipid oxidation are one of the main factors inducing protein oxidation.The effects of oxidation treatment with malondialdehyde(MDA)on the immunoreactivity of amandin and its digestion were studied.Materials and Methods:The rabbit IgG binding ability of amandin was analyzed by western blotting,and the changes in amandin oxidation and immunoreactivity during digestion of amandin with different degrees of oxidation were investigated in combination with an almond allergen enzyme-linked immunosorbent assay kit.Alteration of linear epitopes of amandin by oxidation was investigated by liquid chromatography-tandemmass spectrometry(LC-MS/MS).Results:The results showed that the immunoreactivity of amandin was significantly reduced after 1 mmol/L MDA and 100 mmol/L MDA treat-ment.However,the 1 mmol/L MDA treatment was owing to cleavage of linear epitope peptide in amandin and oxidation of the active amino acid.The 100 mmol/L MDA treatment was due to aggregation of amandin and significant decrease in its solubility.Oxidation also reduced di-gestibility of amandin and significantly affected immunoreactivity during digestion.LC-MS/MS also identified four oxidation-prone methionine sites(aa 264-274,298-308,220-240,and 275-297)in gamma conglutinin 1.Conclusions:MDA treatment reduced the immunoreactivity of amandin.MDA treatment also led to protein aggregation,which slowed down the digestion of amandin and altered the immunoreactivity of amandin during digestion.

关 键 词：OXIDATION amandin IMMUNOREACTIVITY DIGESTION LC-MS/MS 

分 类 号：TS20[轻工技术与工程—食品科学]