超声辅助提取日本荚蒾果实总三萜工艺优化及其抗氧化活性  

Optimization of Ultrasonic-Assisted Extraction and Antioxidant Analysis of Total Triterpenes from Viburnum japonicum Fruit

作　　者：常超 蔡海莺[1,2] 陈瑞达 刘畅 赵敏洁[2] 孙蓉[1] 陈献志 CHANG Chao;CAI Haiying;CHEN Ruida;LIU Chang;ZHAO Minjie;SUN Rong;CHEN Xianzhi(College of Biology and Chemical Engineering,Zhejiang University of Science and Technology,Hangzhou 310023,Zhejiang,China;School of Biosystem Engineering and Food Science,Zhejiang University,Hangzhou 310058,Zhejiang,China;Station of Linhai Forestry Technology Promotion and Field Nursery Tourism Service,Taizhou 317000,Zhejiang,China)

机构地区：[1]浙江科技学院生物与化学工程学院,浙江杭州310023 [2]浙江大学生物系统工程与食品科学学院,浙江杭州310058 [3]临海市林业技术推广和场圃旅游服务总站,浙江台州317000

出　　处：《食品研究与开发》2023年第15期90-97,共8页Food Research and Development

基　　金：国家自然科学基金项目(31972079);浙江省自然科学基金项目(LGF20E030001)。

摘　　要：为高效提取日本荚蒾果实中总三萜,采用超声辅助提取和响应面分析联用方法得到最优提取方案,并分析提取物的抗氧化活性。结果显示,乙醇浓度40%、料液比1∶12.5(g/m L)、超声温度50℃、超声时间74 min的工艺条件下总三萜得率为9.07%,与模型预测值9.16%有良好的拟合性。4个因素影响日本荚蒾中总三萜提取率的顺序为超声时间>料液比>乙醇浓度>超声温度。提取物抗氧化活性检测结果显示,自由基清除率随总三萜质量浓度增加有所升高并趋于稳定,总三萜质量浓度提高到2.5 mg/mL时,DPPH自由基清除率稳定在89%,ABTS+自由基清除率稳定在78%。To efficiently extract total triterpenes from Viburnum japonicum fruits,ultrasonic-assisted extraction and response surface analysis were used to obtain the optimal extraction scheme,and the antioxidant activity of the extract was analyzed.The experimental results showed that under the conditions of ethanol concentration of 40%,solid-to-liquid ratio of 1∶12.5(g/mL),ultrasonic temperature of 50℃,and ultrasonic duration of 74 min,the total triterpene yield was 9.07%,which agreed with the predicted value of 9.16%.The order of four factors affecting the extraction rate of total triterpenes in V.japonicum was ultrasonic duration>solid-to-liquid ratio>ethanol concentration>ultrasonic temperature.The antioxidant activity of the extract showed that the free radical scavenging rate increased with the increase in total triterpene concentration and was finally stabilized.When the total triterpene concentration increased to 2.5 mg/mL,the free radical scavenging rate of DPPH was 89%and that of ABTS+was 78%.

关 键 词：日本荚蒾果实 总三萜 超声辅助提取 工艺优化 抗氧化 

分 类 号：TS201.2[轻工技术与工程—食品科学]