萱草杂交后代及其亲本香气成分分析  

Analysis of Flora Volatiles of the Hybrids and Their Parents in Daylily(Hemerocallis)

作　　者：焦芳[1,2] 孙国峰 李丽芳[2] 张金政[1] JIAO Fang;SUN Guofeng;LI Lifang;ZHANG Jinzheng(Key Laboratory of Plant Resources and Beijing Botanical Garden,Institute of Botany,the Chinese Academy of Sciences,Beijing 100093,China;Beijing Florascape Co.,Ltd.,Beijing 100035,China)

机构地区：[1]中国科学院植物研究所,北方资源植物重点实验室,北京100093 [2]北京市花木有限公司,北京100035

出　　处：《园艺学报》2023年第7期1495-1504,共10页Acta Horticulturae Sinica

基　　金：北京市科技计划项目(D17110900710000)。

摘　　要：以萱草(HemerocallisL.)3个杂交组合的18个优选F_(1)代植株及其亲本为试材,利用顶空固相微萃取结合气相色谱—质谱(HS-SPME-GC-MS)技术对其香气成分进行定性和定量测定;对决定萱草花香的两种主要香气成分(E)-β-罗勒烯和芳樟醇及香气成分总量进行分析;为明确F_(1)代个体香气成分的组成和含量是接近父本还是母本,对其香气成分的相对释放率做One-waySIMPER分析。结果表明:(1)3个杂交组合后代的香气成分组成和含量均与其亲本相似,母本相同时,父本的香气成分相对释放率越高,其后代香气成分的相对释放率也越高;(2)杂交亲本的主要香气成分为(E)-β-罗勒烯时,则后代个体中的(E)-β-罗勒烯含量也较高,含量和香气成分总量均介于亲本之间;杂交亲本的香气成分同时含有(E)-β-罗勒烯和芳樟醇时,则杂交后代个体中(E)-β-罗勒烯的含量可能高于亲本或与亲本接近,而芳樟醇含量介于亲本之间,香气成分总量高于两个亲本。(3)浓香型萱草野生种与没有明显香味的萱草品种杂交,能产生花香清淡的F_(1)代个体,是培育香花型萱草品种的良好种质资源。Eighteen F_(1) hybrids from three cross combinations of Hemerocallis and their parents were used as materials,and the floral volatiles were qualitatively and quantitatively determined by headspace solid-phase extraction combined with gas chromatography-mass spectrometry(HS-SPME-GC-MS).Relative release rate of the two main flora volatiles which decide the fragrance and the total floral volatiles of the tested materials were analyzed.One-way SIMPER analysis was performed to figure out the percentage of similarity with parents.Result showed that:(1)The composition and content of the floral volatiles of the F_(1) hybrids of the three hybrid combinations were similar to their parents,when the female parent is the same,the higher the relative release rate of the floral volatiles of the male parent,the higher the relative release rate of the floral volatiles of its F_(1) hybrids;(2)If the main floral volatile of the hybrid parents were(E)-β-ocimene,the F_(1) hybrids also contained higher amount of(E)-β-ocimene and the content Jiao Fang,Sun Guofeng,Li Lifang,Zhang Jinzheng.Analysis of flora volatiles of the hybrids and their parents in daylily(Hemerocallis).1496 Acta Horticulturae Sinica,2023,50(7):1495-1504.of which was between the two parents,and the total amount of flora volatiles was also between the two parents;if the main flora volatiles of the parents contained both(E)-β-ocimene and linalool,the content of(E)-β-ocimene in some of the F_(1) hybrids were higher than or similar to that of the parents,while the content of linalool was between the two parents,the total amount of floral volatiles was basically higher than that of the two parents;(3)Wild species with intense fragrance crossed with cultivars with no significant fragrance could breed fragrant F_(1) hybrids,so they are good germplasm resources to breed fragrant daylilies.

关 键 词：萱草 杂交 香气成分 HS-SPME-GC-MS 

分 类 号：S682.19[农业科学—观赏园艺]