Intervention effects and related mechanisms of glycyrrhizic acid on zebrafish with Hirschsprung-associated enterocolitis  

作　　者：Ming-Kun Liu Ying-Jian Chen Fei Chen Zhi-Xiong Lin Zi-Cheng Zhu Yu Lin Yi-Fan Fang Dian-Ming Wu 

机构地区：[1]Department of Pediatric Surgery,Fujian Maternity and Child Health Hospital,College of Clinical Medicine for Obstetrics&Gynecology and Pediatrics,Fujian Medical University,Fuzhou 350001,Fujian Province,China [2]Department of Pediatric Surgery,Fujian Children’s Hospital(Fujian Branch of Shanghai Children’s Medical Center),College of Clinical Medicine for Obstetrics&Gynecology and Pediatrics,Fujian Medical University,Fuzhou 350000,Fujian Province,China [3]Department of Pediatric Surgery,Fujian Children’s Hospital,Fuzhou 350001,Fujian Province,China [4]Department of Clinical Medicine,Fujian Medical University,Fuzhou 350001,Fujian Province,China

出　　处：《World Journal of Gastrointestinal Surgery》2023年第7期1317-1330,共14页世界胃肠外科杂志（英文版）（电子版）

基　　金：Joint Funds for the Innovation of Science and Technology,Fujian Province,No.2020Y9139;Startup Fund for Scientific Research,Fujian Medical University,No.2019QH1141.

摘　　要：BACKGROUND The prevention and treatment of Hirschsprung-associated enterocolitis(HAEC)is a serious challenge in pediatric surgery.Exploring the mechanism of HAEC is conducive to the prevention of this disease.AIM To explore the possible mechanism of glycyrrhizic acid(GA)and its therapeutic effect on HAEC.METHODS We developed a model of enteritis induced by trinitrobenzenesulfonic acid(TNBS)in zebrafish,and treated it with different concentrations of GA.We analyzed the effect of GA on the phenotype and inflammation of zebrafish.RESULTS After treatment with TNBS,the area of the intestinal lumen in zebrafish was significantly increased,but the number of goblet cells in the intestinal lumen was significantly reduced,but these did not increase the mortality of zebrafish,indicating that the zebrafish enteritis model was successfully developed.Different concentrations of GA protected zebrafish with enteritis.In particular,high concentrations of GA were important for the prevention and control of HAEC because it significantly reduced the intestinal luminal area,increased the number of goblet cells in the intestinal lumen,and reduced the levels of interleukin(IL)-1βand IL-8.CONCLUSION GA significantly reduced the intestinal luminal area,increased the number of intestinal goblet cells,and decreased IL-1βand IL-8 in zebrafish,and is important for prevention and control of HAEC.

关 键 词：Hirschsprung-associated enterocolitis High mobility group box 1 protein Toll-like receptor 4 INTERLEUKIN-1Β INTERLEUKIN-8 Glycyrrhizic acid 

分 类 号：R574.62[医药卫生—消化系统]