长茎葡萄蕨藻中β-1,3-木聚糖的提取工艺优化及单糖组成分析  

Optimization of Extraction Process and Analysis of Monosaccharide Composition ofβ-1,3-xylan from Caulerpa lentillifera

作　　者：刘倩 晋文慧[1,3] 矫浩田 谢全灵 张怡评 洪专 赵元晖 LIU Qian;JIN Wenhui;JIAO Haotian;XIE Quanling;ZHANG Yiping;HONG Zhuan;ZHAO Yuanhui(Technical Innovation Center for Utilization of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,China;Sanya Institute of Oceanography,Ocean University of China,Sanya 572024,China;Xiamen Ocean Vocational College,Xiamen 361002,China)

机构地区：[1]自然资源部第三海洋研究所,海洋生物资源开发利用工程技术创新中心,福建厦门361005 [2]中国海洋大学三亚海洋研究院,海南三亚572024 [3]厦门海洋职业技术学院,福建厦门361000

出　　处：《食品工业科技》2023年第16期210-217,共8页Science and Technology of Food Industry

基　　金：福建省科技计划项目(2020N0027);自然资源部第三海洋研究所基本科研业务费专项资金项目(2022006,2023001);厦门市科技计划项目(3502Z20221036);厦门海洋研究开发院共建项目(K230101)。

摘　　要：为了优化β-1,3-木聚糖的提取工艺并分析其单糖组成,试验对常见经济藻类进行筛选,以长茎葡萄蕨藻为主要原料,采用碱提方法,以提取时间、料液比和NaOH浓度为考察因素,在单因素优化的基础上设计三因素三水平正交试验,研究粗木聚糖的最优提取条件,并采用特异性酶解方法分析粗木聚糖的单糖组成成分。结果表明,各因素对粗木聚糖提取率影响顺序依次为:提取时间>料液比>NaOH浓度。最佳提取工艺为:提取时间3 h、NaOH浓度为2.5 mol·L^(-1)、料液比1:100 g·mL^(-1),粗木聚糖得率为28.1%±0.6%。采用薄层色谱法对酶解产物进行测定,表明从长茎葡萄蕨藻中提取的粗木聚糖为β-1,3-木聚糖,并明确其单糖组分为β-1,3-木糖。This study screened several common economic algae to optimize the extraction process ofβ-1,3-xylan and analyze its monosaccharide composition.The extraction process of crude xylan was optimized by alkaline extraction method,and the monosaccharide composition of crude xylan was identified by a specific enzymatic method using Caulerpa lentillifera as the main raw material.The effect of extraction time,solid-liquid ratio and NaOH concentration on the yield of crude xylan was optimized with a single factor tests followed by three-three level orthogonal experiment to study the optimal extraction conditions of crude xylan.The results showed that the extraction rate of crude xylan was influenced by the following factors:Extraction time>material-to-liquid ratio>NaOH concentration.The optimum extraction process was:Extraction time of 3 h,NaOH concentration of 2.5 mol·L^(−1),feed-to-liquid ratio of 1:100 g·mL^(−1),and the yield of crude xylan was 28.1%±0.6%. The enzymatic products were determined by thin-layer chromatography, which showed that the crude xylan extracted from Caulerpa lentillifera was β-1,3-xylan and the monosaccharide fraction was β-1,3-xylose.

关 键 词：海藻 长茎葡萄蕨藻 β-1 3-木聚糖 碱提取 提取工艺 β-1 3-木聚糖酶(Xyl3088) 

分 类 号：TS201.1[轻工技术与工程—食品科学]