碱韭总黄酮含量测定方法优选  

Optimization of Determination Method of Total FlavonoidsContent in Allium polyrhizum Turcz. ex Regel

作　　者：高雪峰 高健 杨昱萍 门中华 董贵成 赵鹏 GAO Xuefeng;GAO Jian;YANG Yuping;MEN Zhonghua;DONG Guicheng;ZHAO Peng(School of Biological Science and Technology,Baotou Teachers College,Inner Mongolia University of Science and Technology,Baotou 014030,China;College of Life Science,Inner Mongolia Agricultural University,Hohhot 010011,China)

机构地区：[1]内蒙古科技大学包头师范学院生物科学与技术学院,内蒙古包头014030 [2]内蒙古农业大学生命科学学院,内蒙古呼和浩特010011

出　　处：《食品安全导刊》2023年第21期70-75,79,共7页China Food Safety Magazine

基　　金：中央引导地方科技发展资金项目(2020ZY0025);内蒙古自然科学基金项目(2020MS03071);内蒙古自治区第十二批“草原英才”创新创业人才团队(内人才发[2023]1号);2022年度“草原英才”滚动支持人才项目(内组通字[2022]15号);内蒙古自治区人才开发基金2022年度(总第二十二批)高层次人才个人项目(内人社办发[2022]110号);包头师范学院专创融合课程建设项目(BSSCKC21003);国家级大学生创新创业训练计划项目(202210131001);自治区级大学生创新创业训练计划项目(S202210131015)。

摘　　要：目的:筛选适用于测定碱韭总黄酮含量的试验方法,并对不同采集年份、不同部位碱韭总黄酮进行分析评价。方法:以芦丁为对照品,碱韭叶为试验材料,对比NaNO_(2)-Al(NO_(3))_(3)-NaOH比色法、AlCl_(3)比色法、直接测定法对碱韭总黄酮含量测定的影响。然后对AlCl_(3)比色法测定条件(显色时间、AlCl3加入量、显色温度、p H值和乙醇的浓度)进行了优化。结果:选用AlCl_(3)比色法测定的结果准确可靠,采用优化后的AlCl_(3)比色法测定条件测得碱韭总黄酮主要存在于叶和花中,平均含量为(38.60±5.79)mg·g^(-1)。结论:碱韭具有较高的黄酮应用价值,可作为食品黄酮类添加剂和营养保健食品开发利用。Objective:To screen test methods suitable for determining the total flavonoid content of Allium polyrhizum Turcz.ex Regel,and to analyze and evaluate the totalflavonoid content of Allium polyrhizum Turcz.ex Regel in different collection years and parts.Method:Using rutin as the reference material and Allium polyrhizum Turcz.ex Regel leaves as the experimental material,the effects of NaNO_(2)-Al(NO_(3))_(3)-NaOH colorimetric method,AlCl_(3) colorimetric method,and direct determination method on the determination of totalflavonoid content in Allium polyrhizum Turcz.ex Regel were compared.Then,the determination conditions of the AlCl_(3) colorimetric method(color development time,AlCl_(3) addition amount,color development temperature,pH value,and ethanol concentration)were optimized.Result:The results obtained by using AlCl_(3) colorimetric method were accurate and reliable.The optimized AlCl_(3) colorimetric method was used to determine the conditions under which the total flavonoids of alkali leeks mainly exist in leaves andflowers,with an average content of(38.60±5.79)mg·g^(-1).Conclusion:Allium polyrhizum Turcz.ex Regel has highflavonoid application value and can be used as a foodflavonoid additive and nutritional health food development and utilization.

关 键 词：碱韭 AlCl_3比色法 总黄酮 

分 类 号：TS207.3[轻工技术与工程—食品科学]