基于近红外光谱技术快速分析大米肽的主要成分  

Rapid Analysis of Main Components of Rice Peptide Based on Near Infrared Spectroscopy

作　　者：黄莹 赵彤 黄晶 孙鑫 陈曦 杨晓 HUANG Ying;ZHAO Tong;HUANG Jing;SUN Xin;CHEN Xi;YANG Xiao(Anti-aging Nutrition&Health Research and Development Center of China Health Promotion Foundation,Wuhan 430100)

机构地区：[1]中国健康促进基金会抗衰老营养与健康研发中心,武汉430100

出　　处：《食品工业》2023年第7期295-300,共6页The Food Industry

摘　　要：为实现大米肽主要成分的快速分析,采用近红外光谱(NIRS)技术,测定105个大米肽样品的主要成分,包括蛋白质、多肽、水分、灰分和脂肪的含量,并采集各样品的近红外光谱,建立基于NIRS技术的大米肽主要成分的检测方法。结果表明,大米肽样品主要成分的含量范围为蛋白质29.73%~103.00%,多肽25.88%~78.97%,水分3.71%~8.34%,灰分0.28%~5.63%和脂肪0.01%~0.45%,数据范围可用于NIRS技术定量建模。根据偏最小二乘法(partial least squares,PLS)分别建立大米肽主要成分的最优模型,结果发现模型预测值和实测值的相关系数分别为蛋白质0.98326,多肽0.98573,水分0.96886,灰分0.97860和脂肪0.97651,说明各主要成分预测值和实测值的相关性较高。对模型进行外部验证,各主要成分的实测值和预测值相比均无显著性差异(均为P>0.05),说明模型具有较好的预测能力。因此,采用NIRS分析技术能够准确、快速地测定大米肽主要成分的含量。In order to realize the rapid analysis of the main components of rice peptide,by NIRS technology,the main components of 105 rice peptide samples were determined,including protein,polypeptide,moisture,ash and fat mass content,and the near-infrared spectra of each sample was collected.A method for the determination of main components of rice peptides based on NIRS was established.The results showed that the mass content range of the main components of rice peptide samples were protein 29.73%-103.00%,polypeptide 25.88%-78.97%,water 3.71%-8.34%,ash 0.28%-5.63%and fat 0.01%-0.45%.The data range met the needs of quantitative modeling of NIRS.The LPS was used to establish the optimal models of the main components of rice peptides,the correlation coefficients between the predicted value and the measured value were protein 0.98326,polypeptide 0.98573,water 0.96886,ash 0.97860 and fat 0.97651,respectively,indicating that the predicted value of each major component had a high correlation with the measured value.The external validation of the model showed that there were no significant differences between the measured value and the predicted value of the major components(both P>0.05),indicating that the model had good prediction ability.Therefore,the mass content of the main components of rice peptide could be accurately and rapidly determined by NIRS.

关 键 词：近红外光谱 大米肽 蛋白质 多肽 水分 灰分 脂肪 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.33[轻工技术与工程—食品科学与工程]