2020年上海市市售食品中食源性单核细胞增生李斯特菌基因分型分析  

Analysis on genotyping of foodborne Listeria monocytogenes in commercially available food ofShanghai in 2020

作　　者：王筱 丁锦 崔琪奇 周雨晴 潘丽峰[1] 郝莉鹏 WANG Xiao;DING Jin;CUI Qiqi;ZHOU Yuqing;PAN Lifeng;HAO Lipeng(Microbiological Testing Department,Pudong New Area Center for Disease Control and Prevention,Shanghai,200136,China)

机构地区：[1]上海市浦东新区疾病预防控制中心微检科,上海200136

出　　处：《职业与健康》2023年第13期1774-1778,共5页Occupation and Health

基　　金：2021—2022年上海市疾病预防控制青年骨干人才培养项目(21QNGG18);上海市浦东新区卫生健康公共卫生高原学科—病原微生物检验项目(PWYggy2021-01)。

摘　　要：目的 分析2020年上海市市售食品中单核细胞增生李斯特菌(listeria monocytogenes,LM)的基因分型特征。方法 采用脉冲场凝胶电泳(plused-field gel electrophoreisi,PFGE)和全基因组测序(whole genome sequencing,WGS)对2020年所获得的62株菌株进行分析。结果 62株单核细胞增生李斯特菌的PFGE聚类分析得到17种型别,1型为优势带别,通过全基因组测序数据分析多位点序列分型(multilocus sequence typing,MLST)得到12种序列型(sequence type,ST)型、ST9为优势型别,均携带磷霉素抗性基因(fosfomycin antibiotic gene,fosX)、毒力岛1(listeria pathogenicity island-1,LIPI-1)、毒力岛2(listeria pathogenicity island-2,LIPI-2)、毒力岛3(listeria pathogenicity island-3,LIPI-3)及其他毒力基因,部分菌株携带氨基糖苷类抗性基因(aminoglycoside antibiotic gene,ANT(6)-la)、大环内酯类抗性基因(macrolide antibiotic gene,ErmB)、甲氧苄氨嘧啶抗性基因(trimethoprim resistant gene,dfrG)、四环素抗性基因(tetracycline resistant gene,tetM)、林可酰胺抗性基因(lincosamide antibiotic gene,lin)、氯霉素抗性基因(chloramphenicol antibiotic gene,cat)等,出现对四环素、氯霉素、红霉素耐药的菌株,血清型分布为1/2a、1/2b、1/2c和4b型。结论 2020年上海市市售食品中食源性单增李斯特菌的分子分型具有多态性,存在多种致病型别,同源性比较效果MLST>PFGE>血清型,携带多种毒力基因,部分菌株出现耐药特征。Objective To study the genotyping characteristics of Listeria monocytogenes(LM)in commercially available food of Shanghai in 2020.Methods Totally 62 strains in 2020 were analyzed by pulsed-field gel electrophoreisi(PFGE)and whole genome sequencing(WGS).Results PFGE clustering analysis of 62 strains of LM identified 17 types and the dominant band was type 1.The multilocus sequence typing(MLST)of WGS data showed 12 sequence type(ST)types and the dominant type was ST9.All carried fosfomycin antibiotic gene(fosX),listeria pathogenicity island-1(LIPI-1),listeria pathogenicity island-2(LIPI-2),listeria pathogenicity island-3(LIPI-3)and other virulence gene.Some strains carried aminoglycoside antibiotic gene[ANT(6)-la],macrolide antibiotic gene(ErmB),trimethoprim resistant gene(dfrG),tetracycline resistant gene(tetM),lincosamide antibiotic gene(lin),and chloramphenicol antibiotic gene(cat).There were strains resistant to tetracycline,chloramphenicol and erythromycin,with serotypes of 1/2a,1/2b,1/2c and 4b.Conclusion The molecular typing of foodborne Listeria monocytogenes in commercially available food of Shanghai in 2020 has high diversity,there are many pathogenic types,the comparison effect of homology is MLST>PFGE>serotype,carring a variety of virulence genes,and ome strains show drug resistance characteristics.

关 键 词：单核细胞增生李斯特菌 脉冲场凝胶电泳 全基因组测序 耐药 

分 类 号：R155.5[医药卫生—营养与食品卫生学]