Schisandra B inhibits proliferation, migration and invasion of bladder cancer by regulating the Wnt/β‑catenin signaling pathway  

作　　者：XIANG Ling‑bao ZHU Yi ZUO Ling LIU Hong‑wei 

机构地区：[1]Laboratory of Urology,Affiliated Hospital of Guangdong Medical University,Zhanjiang 524001,China [2]Department of Traditional Chinese Medicine,the Second Affiliated Hospital of Guangdong Medical University,Zhanjiang 524001,China

出　　处：《Journal of Hainan Medical University》2023年第8期8-14,共7页海南医学院学报（英文版）

基　　金：Guangdong Provincial Basic and Applied Basic Research Fund(No.2022A1515012195);Guangdong Provincial Bureau of Traditional Chinese Medicine Research Project(No.20211221,No.20222099);Guangdong Medical University Research Fund(No.4SG20158G)。

摘　　要：Objective:To investigate the effects of Schisandra B on proliferation,migration,invasion of bladder cancer and to further investigate its molecular mechanism.Methods:Bladder cancer cells were subjected to different concentrations of Schisandra B solution(0,20,40,80μmol/L).CCK-8 assay was used to detect the effect of schisandra B on bladder cancer cell proliferation.Transwell migration assay and wound healing assay were used to detect the effect of Schisandra B on the migration of bladder cancer cells.Transwell invasion assay was used to detect the effect of schisandra B on invasion ability of bladder cancer cells.The expression levels of intracellularβ-catenin and c-myc protein were measured by western blot.Results:Schisandra B inhibited the proliferation of T24 and UM-UC-3 cells in a concentration and time dependent manner(P<0.05).The rate of wound healing and number of migration and invasion cells decreased with the increase of Schisandra B concentration(P<0.05).The expression ofβ-catenin and c-myc decreased after treatment with Schisandra B in bladder cancer cells(P<0.05).Conclusion:Schisandra B can inhibit the proliferation,migration and invasion of human bladder cancer T24 and UM-UC-3 cells,and the main mechanism for its inhibitory effect may be related to the inactivation of the Wnt/β-catenin signaling pathway.

关 键 词：Schisandra B Bladder cancer PROLIFERATION Migration and invasion Wnt/β-catenin pathway 

分 类 号：R737.14[医药卫生—肿瘤]