山竹壳膳食纤维结合酚提取工艺的优化及抗氧化活性分析  

Extraction process optimization and antioxidant activity analysis of Mangosteen(Garcinia mangostana)peel dietary fibre bound phenols

作　　者：赖梦婷 刘晓珍 陈宏著 李福香 闫景坤 李琳[2] 赵力超[1] LAI Meng-Ting;LIU Xiao-Zhen;CHEN Hong-Zhu;LI Fu-Xiang;YAN Jing-Kun;LI Lin;ZHAO Li-Chao(Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods,College of Food Science,South China Agricultural University,Guangzhou 510640,China;Engineering Research Center of Health Food Design&Nutrition Regulation,Dongguan Key Laboratory of Typical Food Precision Design,China National Light Industry Key Laboratory of Healthy Food Development and Nutrition Regulation,School of Life and Health Technology,Dongguan University of Technology,Dongguan 523808,China)

机构地区：[1]华南农业大学食品学院,广东省功能食品活性物重点实验室,广州510640 [2]东莞理工学院生命健康技术学院,中国轻工业健康食品开发与营养调控重点实验室,东莞市特色食品精准设计重点实验室,食品营养健康工程与智能化加工研究中心,东莞523808

出　　处：《食品安全质量检测学报》2023年第13期33-39,共7页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(81803193);广东省自然科学基金项目(2018A030310033);东莞理工学院科研启动专项经费项目(GC300502-33);东莞理工学院高层次人才(创新团队)科研启动项目(KCYCXPT2017007);广东省创新强校创新团队项目(2021KCXTD035)。

摘　　要：目的对比酸法、碱法和酶法水解对山竹壳膳食纤维结合酚(bound polyphenols in mangosteen peel,MDF-PP)的提取效果,并考查其抗氧化性。方法首先依次采用α-高温淀粉酶、木瓜蛋白酶和糖化酶对山竹壳进行酶解,得到膳食纤维(mangosteen peel dietary fibre,MDF)。随后分别采用酸法水解、碱法水解和酶法进行MDF-PP提取,并分别测定了所得MDF-PP的1,1-二苯基-2-三硝基苯肼自由基清除率,对MDF-PP得率最高、抗氧化性最强的提取方法进行工艺优化。结果碱法水解提取MDF-PP效果最好,其所得MDF-PP抗氧化活性最强。最佳碱法水解提取工艺条件为NaOH浓度为24 moL/L、碱水解时间为4 h、液固比为30:1 mL/g。在该条件下MDF-PP得率为(88.44±1.10)mg GAE/10 g DW,与预测结果接近。结论建立的响应面模型良好,可用于预测碱法提取山竹壳时MDF-PP得率,MDF-PP具有较好的抗氧化性。Objective To compare the extraction effects of bound polyphenols in mangosteen peel(MDF-PP)by acid,alkali and enzymatic hydrolysis and investigate its antioxidant activity.Methods Mangosteen peel dietary fibre(MDF)was obtained by enzymolysis of the shell withα-high temperature amylase,papain and saccharifying enzyme.MDF-PP was extracted by acid hydrolysis,alkali hydrolysis and enzymatic method,and the free radical removal rate of 1,1-diphenyl-2-picrylhydrazyl of the obtained MDF-PP was determined respectively.The extraction method of MDF-PP with the highest yield and the strongest antioxidant activity was optimized.Results Alkaline hydrolysis was the best method to extract MDF-PP and the antioxidant activity of MDF-PP was the strongest.The optimum extraction condition of the alkaline hydrolysis method was:24 moL/L NaOH,4 h alkaline hydrolysis,and the liquid-solid rate of 30:1 mL/g.The extraction ratetof the dietary fibre bound phenols under these extraction conditions was(88.44±1.10)mg GAE/10 g DW while the content of the MDF-PP was fairly closed to the predicted values.Conclusion The established response surface model is good and can be used to predict the yield of MDF-PP in the extraction of mangosteen peel by alkali method.MDF-PP has good antioxidant activity.

关 键 词：山竹壳 膳食纤维 结合酚 抗氧化活性 响应面 碱法水解 

分 类 号：TS201.2[轻工技术与工程—食品科学]