An AND-Gate DNA Nanodevice for Dual Protein-Mediated,Tumor-Specific Imaging  

作　　者：Zetan Fan Fangzhi Yu Mingming Yu Lele Li 

机构地区：[1]CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, 100190 China [2]Beijing Municipal Pollution Source Management Center, Beijing, 100089 China [3]Green Catalysis Center and College of Chemistry, Zhengzhou University, Zhengzhou, Henan, 450001 China

出　　处：《Chinese Journal of Chemistry》2023年第12期1437-1442,共6页中国化学（英文版）

基　　金：financially supported by the Beijing Natural Science Foundation(JQ20005).

摘　　要：Sensitive and specific imaging of tumor-associated biomarkers offers an effective way for cancer diagnosis.However,most of the reported imaging probes are limited to the targeting of a single biomarker,which may impair imaging precision.Here,we report an aptamer-based,AND-gate DNA nanodevice that relies on the sequential processing of two tumor-associated proteins to produce a specific fluorescent signal for precise tumor imaging.The DNA nanodevice integrates an AS1411 aptamer to bind cell surface nucleolin for tumor targeting and a DNA sensor to respond to intracellular apurinic/apyrimidinic endonuclease 1 for activatable imaging.We evaluated the performance of the system in both living cells and a tumor-bearing mouse model,and demonstrated its capability in tumor cell-specific imaging.We anticipate that this strategy will accelerate the design of multivariate protein-stimulated probes for tumor diagnosis.

关 键 词：APTAMER DNA nanodevice Protein Sensor BIOIMAGING 

分 类 号：R730[医药卫生—肿瘤] TB383.1[医药卫生—临床医学]