Rabbit models of dry eye disease: comparative analysis  

作　　者：Vytautas Baranauskas Julija Daukantaitė Saulius Galgauskas 

机构地区：[1]Vilnius University Institute of Biochemistry,Life Science Centre,Sauletekio av 7,Vilnius,LT 10257,Lithuania [2]Vilnius University Faculty of Medicine,M.K.Čiurlionio str.21,Vilnius,LT 03101,Lithuania

出　　处：《International Journal of Ophthalmology(English edition)》2023年第8期1177-1185,共9页国际眼科杂志（英文版）

摘　　要：AIM:To report ocular changes in rabbits after the implementation of three different induction methods to create dry eye(DE)conditions and provides evidence of DErelated disease evolution.METHODS:Experimental methods were divided into 3 models.The first model used involved triple injection of complete Freund’s adjuvant,50µL each,also called the meibomian gland dysfunction(MGD)model.In the second model,DE conditions were created by the resection of nictitating membranes(NM),Harderian glands(HG),and main lacrimal glands(LG),also called the LGR model.The third model involved the topical administration of benzalkonium chloride(BAK)0.1%solution.The Schirmer test,ocular surface staining with fluorescein,and tear breakup time tests were implemented before and after excision.After euthanasia,the ocular tissues were dissected.Cornea,conjunctiva,and meibomian glands were treated with periodic acid–Schiff(PAS)staining and haematoxylin–eosin staining.RESULTS:The MGD model triggered inflammation of meibomian glands.It detected changes in the lipid layer of the tear film.The bilateral resection of NM,HG,and LG reduced the watering layer of the tear film.The topical administration of BAK of 0.1%solution impacted the mucosal layer of the tear film.CONCLUSION:Different changes are observed with different DE syndrome models.The composition of the tear film differ depending on which part of the eye is targeted.More studies need to be done to confirm whether an increased thickness of the cornea has any impact on the DE disease.

关 键 词：ocular surface lid inflammation KERATITIS tear deficiency RABBITS dry eye disease 

分 类 号：R777.34[医药卫生—眼科] R-332[医药卫生—临床医学]