贵州栽培型地方茶树叶片气孔性状全基因组关联分析  

Genome-wide association analysis of stomatal characters of cultivated local tea plants in Guizhou, China

作　　者：白鼎臣 赵支飞 龚雪 刘源 牛素贞 陈正武[5] BAI Dingchen;ZHAO Zhifei;GONG Xue;LIU Yuan;NIU Suzhen;CHEN Zhengwu(College of Tea,Guizhou University,Guiyang 550025,China;College of Cultural Tourism and Regional Development,Guiyang College of Humanities and Technology,Guiyang 550025,China;Guiyang Agricultural Technology Extension Station,Guiyang 550081,China;Institute of Agricultural Bioengineering,Guizhou University,Guiyang 550025,China;Tea Research Institute,Guizhou Academy of Agricultural Sciences,Guiyang 550025,China)

机构地区：[1]贵州大学茶学院,贵州贵阳550025 [2]贵阳人文科技学院文化旅游与区域发展学院,贵州贵阳550025 [3]贵阳市农业技术推广站,贵州贵阳550081 [4]贵州大学农业生物工程研究院,贵州贵阳550025 [5]贵州省农业科学院茶叶研究所,贵州贵阳550025

出　　处：《浙江农业学报》2023年第7期1550-1563,共14页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(32060700);贵州省科技计划项目(黔科合支撑〔2021〕一般126,黔科合服企〔2020〕4016);贵州省教育厅重点领域项目(黔教合KY〔2021〕042)。

摘　　要：通过对贵州栽培型地方茶树气孔性状进行全基因组关联分析(GWAS),找出影响其气孔发育相关基因,为贵州栽培型地方茶树开发利用提供依据。该研究利用简化基因组测序技术(GBS),以253份贵州栽培型地方茶树种质资源为材料,进行全基因组关联分析。结果表明,与5个气孔表型性状有关的SNP位点共关联45个,在下游50 kb的45个SNPS位点范围内共检测到20个基因。其中6个基因与气孔长度(SL)有关,1个基因与气孔宽度(SW)有关,7个基因与气孔面积(SA)有关,2个基因与气孔密度(SD)有关,4个基因与气孔周长(SP)有关,该研究成果有助于对具有复杂遗传背景和丰富生态类型的栽培型茶树资源的开发利用,以便筛选出适应性强、能够促进贵州茶产业品种结构调整的优良茶树品种。In order to provide a basis for the development and utilization of cultivated local tea trees in Guizhou.Through Genome-Wide Association Study(GWAS)on the stomatal traits of cultivated local tea trees in Guizhou,the genes related to stomatal development were identified.This study utilized Simplified Genotyping-by-Sequencing(GBS)technology to conduct GWAS analysis on 253 cultivated local tea tree germplasm resources in Guizhou.The results showed that a total of 45 SNP loci were associated with 5 stomatal phenotypic traits,and a total of 20 genes were detected within the 45 SNPS loci range of 50 kb downstream.Among them,6 genes are related to stomatal length(SL),1 gene is related to stomatal width(SW),7 genes are related to stomatal area(SA),2 genes are related to stomatal density(SD),and 4 genes are related to stomatal perimeter(SP).This research achievement is beneficial for the development and utilization of cultivated tea tree resources with complex genetic backgrounds and rich ecological types,and for screening excellent tea tree varieties with strong adaptability and promoting the adjustment of the tea industry variety structure in Guizhou.

关 键 词：种质资源 栽培型茶树 全基因组关联分析 简化基因组测序 

分 类 号：S571.1[农业科学—茶叶生产加工]