不同碳源对溢油胁迫下小新月菱形藻毒性缓解作用研究  

Effects of different carbon sources on mitigation of toxicity of oil spill to Nitzschia Closterium

作　　者：刘瑀[1] 郝效玉 范惟佳 王国光 LIU Yu;HAO Xiaoyu;FAN Weijia;WANG Guoguang(College of Environmental Science and Engineering,Dalian Maritime University,Dalian 116023,China)

机构地区：[1]大连海事大学环境科学与工程学院,辽宁大连116023

出　　处：《海洋环境科学》2023年第4期534-541,共8页Marine Environmental Science

基　　金：国家科技支撑计划项目(2015BAD17B05);中央高校基本科研业务费专项资金资助项目(3132016332);辽宁省“兴辽英才计划”项目(XLYC2001002)。

摘　　要：为缓解海上溢油胁迫对海洋微藻的毒性效应,添加不同碳源可能是有效途径之一。本文以小新月菱形藻(Nitzschia closterium)为实验材料,通过添加不同浓度的葡萄糖(C_(6)H_(12)O_(6))和碳酸氢钠(NaHCO_(3)),探究在180#燃料油分散液(WAF)胁迫下外源碳源对其毒性效应的缓解作用。结果表明,在不同浓度NaHCO_(3)的作用下,小新月菱形藻的细胞密度和叶绿素a(Chl a)含量增加,脂质过氧化水平降低,培养4天后,脂肪酸组成与正常组基本一致,毒性效应得到明显缓解。不同浓度的C6H12O6均降低了小新月菱形藻Chl a含量,C18:1n-9比例明显增加。低浓度C_(6)H_(12)O_(6)对WAF胁迫下小新月菱形藻毒性效应的缓解作用较小,高浓度C_(6)H_(12)O_(6)加重了WAF的毒性效应,导致了更严重的脂质过氧化。因此,添加适宜浓度的NaHCO_(3)能在一定程度上缓解WAF对小新月菱形藻的毒性效应。In order to alleviate the toxic effects of offshore oil spill stress on Marine microalgae,adding different carbon sources may be one of the effective ways.By adding different concentrations of glucose(C_(6)H_(12)O_6) and sodium bicarbonate (NaHCO_(3)),the alleviation effect of exogenous carbon sources on the toxicity of Nitzschia closterium under 180#fuel oil dispersion (WAF) stress was investigated.Under different concentrations of NaHCO_(3),the cell density and Chl a content increased,while the level of lipid peroxidation decreased.Four days later,the fatty acid composition of N.closterium was basically the same as that of the normal group,and the toxic effect was obviously relieved.Different concentrations of C_(6)H_(12)O_(6) reduced the content of Chl a in N.closterium and increased its C18:1n-9 ratio significantly.Low concentration of C_6H_(12)O_(6) had less alleviation effect on the toxic effect of N.closterium under WAF stress,while high concentration of C_(6)H_(12)O_(6) aggravated the toxic effect of WAF,resulting in more serious lipid peroxidation.Therefore,adding an appropriate concentration of NaHCO_(3) could alleviate the toxic effect of WAF on N.closterium to a certain extent.

关 键 词：小新月菱形藻 WAF 脂质过氧化 脂肪酸组成 

分 类 号：X171.5[环境科学与工程—环境科学]