Scutellarin prevents acute alcohol-induced liver injury via inhibiting oxidative stress by regulating the Nrf2/HO-1 pathway and inhibiting inflammation by regulating the AKT,p38 MAPK/NF-κB pathways  

作　　者：Xiao ZHANG Zhicheng DONG Hui FAN Qiankun YANG Guili YU Enzhuang PAN Nana HE Xueqing LI Panpan ZHAO Mian FU Jingquan DONG 

机构地区：[1]Jiangsu Key Laboratory of Marine Bioresources and Environment/Co-Innovation Center of Jiangsu Marine Bio-Industry Technology/Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening,College of Pharmacy,Jiangsu Ocean University,Lianyungang 222005,China [2]Department of Oncology,the Second People's Hospital of Lianyungang,Lianyungang 222000,China [3]Institute of Neuroscience,the First People's Hospital of Lianyungang,Lianyungang 222000,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2023年第7期617-631,共15页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the Basic Science(Natural Science)Research Project of Higher Education of Jiangsu Province(Nos.21KJB230001 and 21KJB350019);the Open Foundation of Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening(No.HY202101);the Postdoctoral Science Foundation of Lianyungang(No.LYG20220013);the Priority Academic Program Development of Jiangsu Higher Education Institutions of China.

摘　　要：Alcoholic liver disease(ALD)is the most frequent liver disease worldwide,resulting in severe harm to personal health and posing a serious burden to public health.Based on the reported antioxidant and anti-inflammatory capacities of scutellarin(SCU),this study investigated its protective role in male BALB/c mice with acute alcoholic liver injury after oral administration(10,25,and 50 mg/kg).The results indicated that SCU could lessen serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels and improve the histopathological changes in acute alcoholic liver;it reduced alcohol-induced malondialdehyde(MDA)content and increased glutathione peroxidase(GSH-Px),catalase(CAT),and superoxide dismutase(SOD)activity.Furthermore,SCU decreased tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and IL-1βmessenger RNA(mRNA)expression levels,weakened inducible nitric oxide synthase(iNOS)activity,and inhibited nucleotide-binding oligomerization domain(NOD)-like receptor protein 3(NLRP3)inflammasome activation.Mechanistically,SCU suppressed cytochrome P450 family 2 subfamily E member 1(CYP2E1)upregulation triggered by alcohol,increased the expression of oxidative stress-related nuclear factor erythroid 2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)pathways,and suppressed the inflammation-related degradation of inhibitor of nuclear factor-κB(NF-κB)-α(IκBα)as well as activation of NF-κB by mediating the protein kinase B(AKT)and p38 mitogen-activated protein kinase(MAPK)pathways.These findings demonstrate that SCU protects against acute alcoholic liver injury via inhibiting oxidative stress by regulating the Nrf2/HO-1 pathway and suppressing inflammation by regulating the AKT,p38 MAPK/NF-κB pathways.

关 键 词：SCUTELLARIN Oxidative stress Alcoholic liver disease INFLAMMATION 

分 类 号：R285[医药卫生—中药学]