The immunomodulatory effects of bone marrow-derived mesenchymal stem cells on lymphocyte in spleens of aging rats  

作　　者：Zhi-Hong Wang Zhi-Feng Lin Yi-Ting Lai Ling-Ling Ding Huai-Cheng Wang Xing Chen Xiao-Ye Chen Hua-Ke Zeng 

机构地区：[1]Shengli Clinical Medical College of Fujian Medical University,Fuzhou 350001,Fujian,China [2]Department of Hematology,Fujian Provincial Hospital,Fuzhou 350001,Fujian,China [3]Department of Ophthalmology,Fujian Provincial Hospital,Fuzhou 350001,Fujian,China

出　　处：《Biomedical Engineering Communications》2023年第3期1-6,共6页生物医学工程通讯

基　　金：supported by joint funds for the innovation of science and technology,Fujian province(2020Y9027);Fujian Natural Science Foundation(2020J011062);Medical Innovation Project of Fujian Provincial Health Commission(2021CXA004).

摘　　要：Objective:To investigate the effects of bone marrow-derived mesenchymal stem cells(BMSCs)on the proliferation and secretion of IgM,IgG and IL-2 in spleen lymphocytes(L)of aging rats.Methods:BMSCs were isolated by the whole bone marrow adherence method and characterized.A rat model of aging was produced by daily subcutaneous injection of D-galactose into the back of the neck.Rat spleen lymphocyte isolate kit to isolate spleen lymphocytes from aging rats and young rats.In vitro,the co-culture system of BMSCs and aging rats lymphocytes was established,and under the induction of mitogen LPS and ConA,the proliferative activity of lymphocytes in each group was detected by CCK-8 assay,the levels of IgM and IgG in the culture supernatant of each group was detected by ELISA,and the IL-2 radioimmunoassay kits were used to detect the content of IL-2 in the supernatant of each group.Results:(1)The isolated adherent cells showed the characteristics of BMSCs,including spindle-shaped morphology,high expression of CD29,CD44,low expression of CD34 and CD45,and osteogenic/adipogenic ability.(2)Under LPS induction,lymphocyte proliferative activity and secretion of immunoglobulin IgG were reduced in the aging group compared with the young group,and co-culture with BMSCs reversed this trend.(3)Under ConA induction,the IL-2 content of BMSCs co-cultured with aging lymphocytes was higher than that of aging lymphocytes alone(P<0.0001);the IL-2 content of CsA co-cultured with aging lymphocytes was lower than that of aging lymphocytes alone(P<0.0001).Conclusion:BMSCs have immunomodulatory effects on the spleen lymphocytes of aging rats in vitro.

关 键 词：bone marrow-derived mesenchymal stem cell IMMUNOSENESCENCE LYMPHOCYTE IMMUNOMODULATION 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]