Effect of intestinal ischemia/reperfusion injury on leptin and orexin-A levels  

作　　者：LIN Ji YAN Guangtao GAO Xiaoning LIAO Jie HAO Xiuhua ZHANG Kai 

机构地区：[1]Research Laboratory of Biochemistry,General Hospital of PLA,Beijing 100853,China [2]Department of Pediatrics,General Hospital of PLA,Beijing 100853,China [3]Medical and Experimental Analysis Center,General Hospital of PLA,Beijing 100853,China

出　　处：《Frontiers of Medicine》2007年第1期87-92,共6页医学前沿（英文版）

摘　　要：The aim of this paper is to explore the effect of intestinal ischemia/reperfusion(I/R)injury on leptin and orexin-A levels in peripheral blood and central secretory tissues,and to examine the roles of leptin and orexin-A in acute inflammatory responses.An intestinal I/R injury model of rats was made;the rats were grouped according to the time of after 60 min ischemia.Radioimmunoassay was employed to detect the levels of leptin in serum and adipose tissue and orexin-A levels in plasma and hypothalamus.Reverse transcriptase-polymerase chain reaction was used to detect mRNA expressions of adipose leptin and hypothalamus orexin-A.Compared with the levels before the injury,serum leptin in 60 min ischemia/30 min reperfusion(I60pR30p)group decreased and that of I60pR360p group increased.Compared with sham-operation group(sham group)after injury,serum leptin level of I60pR360p group increased,adipose leptin levels of I60pR30p and I60pR90p decreased,and adipose leptin in I60pR360p group increased.After the injury,adipose leptin mRNA expressions of I60pR30p,I60pR240p and I60pR360p increased,whereas that of I60pR150p group decreased as compared with the sham group.There was no significant difference in the protein levels of orexin-A,either between plasma and hypothalamus or between pre-and post-I/R injury.Compared with sham group,hypothalamus orexin-A mRNA expressions of I60pR30p and I60pR90p decreased gradually after the injury,with that of I60pR150p group reaching the lowest,and those of I60pR240p and I60pR360p recovering gradually,although they were still significantly lower than that of sham group.Leptin and orexin-A respond to intestinal I/R injury in a time-dependent manner,with leptin responding more quickly than orexin-A does,and both of them may contribute to the metabolic disorders in acute inflammation.

关 键 词：reperfusion injury INTESTINE LEPTIN OREXIN-A RADIOIMMUNOASSAY reverse transcriptase polymerase chain reaction adipose tissue HYPOTHALAMUS 

分 类 号：R33[医药卫生—人体生理学]