Effects of intracerebroventricular NMDA and non-NMDA receptor agonists or antagonists on general anesthesia of propofol in mice  

作　　者：XU Aijun DUAN Shiming TIAN Yuke 

机构地区：[1]Department of Anesthesiology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China [2]Jiangsu Province Key Laboratory of Anesthesiology,Xuzhou Medical College,Xuzhou 221002,China

出　　处：《Frontiers of Medicine》2007年第2期207-210,共4页医学前沿（英文版）

基　　金：The study was supported by the National Natural Science Foundation of China(Grant No.30672027).

摘　　要：The effects of intracerebroventricular(icv)agonists and antagonists of N-methyl-D-aspartate(NMDA)and alpha-amino-3-hydroxy–5-methyl-4-isoxazole-propionic acid(AMPA)receptors on the general anesthesia of propofol were studied.A total of 144 Kunming mice,male and female with body mass of(22P3)g,were used.Part One of the Experiment:a total of 104 Kunming mice,male and female,were randomly divided into 13 groups.Intracerebroventricular artificial cerebral fluid(aCSF)or different doses of NMDA,AMPA,MK-801 or NBQX was injected immediately after intravenously administered propofol 25 mg/kg and the recovery time following the loss of righting reflex(LORR)was recorded.Part Two of the Experiment:a total of 40 Kunming female mice were divided randomly into 5 groups and injected with icv aCSF or NMDA,AMPA,MK-801 or NBQX after intraperitoneally administered propofol 50 mg/kg.The pain threshold of the mice was then investigated by hot-plate test(HPPT).NMDA(0.05 or 0.075μg,icv)or AMPA(0.05μg,icv)exhibited no effects on the LORR,but NMDA(0.1μg,icv)or AMPA(0.075 or 0.1μg,icv)prolonged the LORR significantly compared with the aCSF group(P<0.05,P<0.01).The LORR of the 2μg MK-801 group had no changes,while those of the 4 or 8μg MK-801 groups were prolonged significantly.The LORR of the 0.5,2 or 4μg NBQX groups were all prolonged significantly.NMDA 0.05μg or AMPA 0.05μg decreased the pain threshold slightly but did not differ in effect compared with the aCSF group;2μg MK-801 or 0.5μg NBQX both increased the pain threshold significantly.Our results indicate that propofol produces general anesthesia partly through an interaction with brain NMDA and AMPA receptors in mice.

关 键 词：PROPOFOL anesthesia general receptors Nmethyl-D-aspartate receptors alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid 

分 类 号：R74[医药卫生—神经病学与精神病学]