Changes of phenotype and function of human CD4^(+)CD25^(-) T cells induced by transfection of Foxp3  

作　　者：Kui WU Yutian BI Yaoli WANG Changzheng WANG 

机构地区：[1]Institute of Respiratory Disease,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China

出　　处：《Frontiers of Medicine》2008年第4期366-369,共4页医学前沿（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.30470772).

摘　　要：The aim of this paper is to explore the effects of transfection of Foxp3 gene on the phenotype and function of naive CD4^(+)T cells.The pMSCV-Foxp3 retroviral vec-tor encoding Foxp3 gene was transduced into the PT67 packaging cell line.Virus-containing supernatant was applied to differentiate CD4^(+)CD25^(-) T cells.The resulting cells were sorted with flow cytometry.The expressions of CD25,CD127,CTLA-4 and the proliferation of trans-fected T cells were examined.The effect of transfected CD4^(+)T cells on the proliferation and cytokine production of CD4^(+)CD25^(-) T cells was examined.Foxp3-gene trans-fected CD4^(+)T cells could express Foxp3 and transfection of Foxp3 gene up-regulated the expressions of CD25 and CTLA-4,but down-regulated CD127 expression.After transfection,the proliferation of CD4^(+)T cells was elimi-nated.Transfected T cells inhibited the proliferation of CD4^(+)CD25^(-) T cells.CD4^(+)CD25^(-) T cells acquired a reg-ulatory phenotype and function after it was transduced with the Foxp3 gene.This suggested a key role of Foxp3 in the generation of CD4^(+)CD25^(+) regulatory T cells.

关 键 词：Forkhead transcription factors FOXP3 pro-tein human T-lymphocytes regulatory RETROVIRIDAE 

分 类 号：R73[医药卫生—肿瘤]