超声辅助双水相提取刺梨黄酮的工艺优化及其对黄嘌呤氧化酶活性的抑制作用  

Optimization of Ultrasonic-assisted Two-aqueous Extraction of Flavonoids from Rosa roxburghii Tratt and Its Inhibitory Effect on Xanthine Oxidase Activity

作　　者：庄乾飞 刘丹丹 陈泽雨 尚祖飞 刘晓燕 马立志 ZHUANG Qianfei;LIU Dandan;CHEN Zeyu;SHANG Zufei;LIU Xiaoyan;MA Lizhi(School of Food and Pharmaceutical Engineering,Guiyang University,Guiyang 550005,China;Guizhou Fruit Processing Engineering Technology Research Center,Guiyang 550005,China;Guizhou Collaborative Innovation Center of Fruit Processing,Storage and Safety Control,Guiyang 550005,China)

机构地区：[1]贵阳学院食品与制药工程学院,贵州贵阳550005 [2]贵州省果品加工工程技术研究中心,贵州贵阳550005 [3]贵州省果品加工、贮藏与安全控制协同创新中心,贵州贵阳550005

出　　处：《食品工业科技》2023年第17期222-230,共9页Science and Technology of Food Industry

摘　　要：本研究采用超声辅助双水相技术(Ultrasound-assisted aqueous two-phase extraction,UA-ATPE)提取刺梨黄酮并探究其体外抑制黄嘌呤氧化酶(Xanthine oxidase,XOD)活性的作用。实验以刺梨黄酮得率为指标,验证单因素及响应面优化黄酮提取工艺,得到最优提取工艺为:在32%C_(2)H_(5)OH-20%(NH_(4))_(2)SO_(4)双水相萃取体系下,当料液比1:59(g/g),超声时间30 min,温度50℃,功率320 W时,黄酮的得率为140.57±1.78 mg/g。且在此条件下,刺梨黄酮体外抑制黄嘌呤氧化酶活性的IC_(50)值为12.72 mg/mL,抑制活性较好。实验证明UA-ATPE提取刺梨黄酮类化合物的工艺优化,有效提高了刺梨黄酮的得率。且提取的刺梨黄酮具有较好的抑制黄嘌呤氧化酶活性的作用,可以作为一种天然的黄嘌呤氧化酶抑制剂。In this study,ultrasound-assisted aqueous two-phase extraction(UA-ATPE)was adopted to extract flavonoids from Rosa roxburghii Tratt and investigated its inhibitory effect of this flavonoid on xanthine oxidase activity in vitro.In this experiment,flavonoids yield of Rosa roxburghii Tratt was taken as the index to verify the single factor and response surface optimization of flavonoids extraction process.The optimal extraction process was obtained as follows:Under the condition of 32%C_(2)H_(5)OH-20%(NH_(4))_(2)SO_(4) two-phase aqueous extraction system,the yield of flavonoids was 140.57±1.78 mg/g when the solid-liquid ratio was 1:59(g/g),the ultrasonic time was 30 min,the temperature was 50℃,and the power was 320 W.Under these conditions,the IC_(50) value of xanthine oxidase was 12.72 mg/mL in vitro,indicating a good inhibitory activity.The experiment showed that the optimized technology of UA-ATPE extraction of flavonoids from Rosa roxburghii Tratt effectively improved the yield of flavonoids from Rosa roxburghii Tratt.And the flavonoids extracted from Rosa roxburghii Tratt had a good effect of inhibiting the activity of xanthine oxidase and could be used as a natural xanthine oxidase inhibitor.

关 键 词：刺梨黄酮 双水相萃取 超声波 黄嘌呤氧化酶 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]