灯盏花乙素对黑色素瘤B16体内外的作用及机制研究  

Effect of Scutellarin on Melanoma B16 in Vitro and in Vivo and Its Mechanism

作　　者：田冲冲 张琦 包小波 陈延绅 TIAN Chongchong;ZHANG Qi;BAO Xiaobo;CHEN Yanshen(Department of Pharmacy,Jiangsu Vocational College of Medicine,Yancheng 224005,China)

机构地区：[1]江苏医药职业学院药学院,江苏盐城224005

出　　处：《食品工业科技》2023年第17期406-412,共7页Science and Technology of Food Industry

基　　金：江苏省高等学校自然科学研究面上项目(18KJB320004);江苏医药职业学院自然科学基金研究重点项目(20214103,20214107)。

摘　　要：本文旨在探究灯盏花乙素(Scutellarin,SCU)抑制黑色素瘤生长的作用途径及机制。采用CCK-8、Transwell实验体外探究SCU对B16肿瘤细胞功能的影响;同时采用C57BL/6小鼠皮下接种B16细胞,建立荷瘤小鼠模型,在体观察SCU对肿瘤生长的作用;采用免疫组织化学方法对肿瘤组织进行形态学观察,分析SCU对肿瘤血管新生的影响;采用流式细胞技术考察SCU对骨髓源性细胞动员的影响。结果显示,SCU在160~640μmol/L浓度范围内可极显著抑制B16细胞的增殖(P<0.01);160、320μmol/L的SCU还能够明显抑制B16肿瘤的迁移和侵袭能力。30 mg/kg SCU及60 mg/kg SCU能够极显著抑制在体肿瘤B16的生长(P<0.01);同时还能极显著减少肿瘤组织的微血管密度(Microvascular density,MVD)(P<0.01)以及减少荷瘤小鼠外周循环血液中血管内皮生长因子受体2阳性(Vascular endothelial growth factor receptor 2 positive,VEGFR2+)骨髓源性细胞(Bone marrow-derived cells,BMDCs)及Gr-1+CD11b+BMDCs的细胞计数(P<0.01)。综上所述,SCU能够抑制B16肿瘤细胞的生长。这一作用可能与其抑制肿瘤细胞功能直接作用有关,也与其抑制BMDCs的动员,从而间接减少了肿瘤血管新生密切相关。The aim of the present study was to clarify the pathway and mechanism involved in the anti-melanona effect of Scutellarin(SCU).CCK-8 and Transwell assays were employed to evaluate the cell proliferation and migratory abilities of B16 in vitro.Then,a B16 tumor-bearing mouse model was established to explore the effect of SCU on tumor growth in vivo.Immunohistochemistry and flow cytometry were also used to examine the effect of SCU on tumor angiogenesis and the mobilization of bone marrow-derived cells,respectively.The results showed that SCU substantially decreased the proliferation of B16 cells at doses of 160,320,and 640μmol/L,respectively(P<0.01).Moreover,the migration and invasion capacity of B16 cells were apparently reduced when treated with 160 and 320μmol/L SCU.Besides,the growth of B16 cells in vivo was remarably slower after administration of 30 and 60 mg/kg SCU(P<0.01).Further immunohistochemistry semi-quantitative results revealed the microvascular density(MVD)of B16 tumor tissues was significantly decreased(P<0.01)and flow cytometry data shown that SCU could lower the cell counts of vascular endothelial growth factor receptor 2 positive(VEGFR2+)bone marrow-derived cells(BMDCs)and Gr-1+CD11b+BMDCs in the peripheral circulating blood of tumor-bearing mice(P<0.01).In conclusion,SCU could inhibit the growth of B16 tumor cells in vitro and in vivo,which may be directly related to its inhibition of tumor cell function,as well as its inhibition of BMDCs mobilization,thus indirectly reducing tumor angiogenesis.

关 键 词：灯盏花乙素 黑色素瘤 迁移侵袭 血管新生 

分 类 号：R739.5[医药卫生—肿瘤]