金抗肽SIF_(4)对大肠杆菌基于细胞壁靶点的非细胞质膜损伤抑菌机理  

Non-Cytoplasmic Membrane Damage Antimicrobial Mechanism of Metal Antimicrobial Peptide SIF_(4) Against Escherichia coli Based on Cell Wall Target

作　　者：李玉珍[1] 肖怀秋[1,2] 刘淼 王琳 曾梦琪 赵谋明 LI Yuzhen;XIAO Huaiqiu;LIU Miao;WANG Lin;ZENG Mengqi;ZHAO Mouming(School of Pharmaceutical and Bioengineering,Hunan Chemical Vocational Technology College,Zhuzhou 412000,China;School of Food Science and Engineering,South China University of Technology,Guangzhou 510641,China)

机构地区：[1]湖南化工职业技术学院制药与生物工程学院,湖南株洲412000 [2]华南理工大学食品科学与工程学院,广东广州510641

出　　处：《食品与生物技术学报》2023年第6期78-83,共6页Journal of Food Science and Biotechnology

基　　金：湖南省自然科学基金科教联合基金项目(2022JJ60046,2021JJ600040)。

摘　　要：为阐明金抗肽SIF_(4)对食源性大肠杆菌基于细胞壁靶点的非细胞质膜损伤抑菌机理,研究了SIF_(4)对细胞壁损伤的影响、与细胞壁脂多糖竞争性结合机理及对细胞壁膜组分影响机理,并运用扫描电镜分析了菌体形貌变化。研究发现,SIF_(4)对菌体细胞壁有破坏作用,在一定浓度范围内,细胞壁受损与SIF_(4)处理时间和处理剂量呈正相关,且组间差异显著(P<0.05);SIF_(4)可与细胞壁脂多糖(LPS)竞争性结合,结合量为256 mg/L或更大时,表现无抑菌活性;傅里叶变换红外光谱(FT-IR)分析发现,SIF_(4)对细胞壁多糖信息区、蛋白质与脂肪酸混合信息区影响明显,揭示细胞壁是潜在抑菌效应靶点;扫描电镜(SEM)分析发现,SIF_(4)可破坏菌体细胞壁膜结构并改变菌体形貌。研究认为,SIF_(4)可基于细胞壁损伤靶点作用于大肠杆菌并实现高效抑菌活性,研究结果可为阐明基于细胞壁靶点的非细胞质膜损伤抑菌机理和食源性大肠杆菌生物防控提供依据。To elucidate the non-cytoplasmic membrane damage antimicrobial mechanism of metal antimicrobial peptide SIF_(4) against foodborne Escherichia coli(E.coli)based on cell wall target,the effect of SIF_(4) on cell wall damage,the competitive binding mechanism of SIF_(4) with cell wall lipopolysaccharide(LPS),and the effect of SIF_(4) on cell wall membrane componenTSwere investigated,and the changes in cell morphology of strain were analyzed using scanning electron microscopy.ResulTSdisplayed that SIF_(4) could destroy the bacterial cell wall.Within a certain concentration range,the damage to the cell wall was positively correlated with the treatment time and treatment dose of SIF_(4),and the difference between groups was significant(P<0.05).SIF_(4) could competitively bind with lipopolysaccharides(LPS),however,no antibacterial activity was observed when the binding amount exceeded 256 mg/L.Fourier transform infrared spectroscopy(FT-IR)analysis revealed that SIF_(4) had a significant impact on cell wall polysaccharide information area,as well as the mixed information area of protein and fatty acid,indicating that cell wall was the potential antimicrobial targets.Scanning electron microscope(SEM)analysis showed that SIF_(4) could destroy the cell wall membrane structure and alter strain morphology.This study suggesTSthat SIF_(4) can exert efficient antimicrobial activity against E.coli based on cell wall damage target.Research resulTScould provide supporTSfor elucidating the mechanism of non-cytoplasmic membrane damage based on cell wall targeTSand biological control of foodborne E.coli.

关 键 词：金抗肽 细胞壁 大肠杆菌 非细胞质膜损伤 抑菌机理 

分 类 号：TS201.3[轻工技术与工程—食品科学]