一起由副溶血性弧菌引起的聚集性腹泻事件的病原特征分析  

Analysis of the pathogenic characteristics of a clustered diarrhea event caused by Vibrio parahaemolyticus

作　　者：吴晓芳[1] 陈莉萍[1] 沈月华[1] 严伟[1] 纪蕾[1] 许向东 WU Xiaofang;CHEN Liping;SHEN Yuehua;YAN Wei;JI Lei;XU Xiangdong(Center for Disease Control and Prevention of Huzhou City,Zhejiang Huzhou 313000,China;Center for Disease Control and Prevention of Nanxun District,Zhejiang Huzhou 313009,China)

机构地区：[1]湖州市疾病预防控制中心,浙江湖州313000 [2]南浔区疾病预防控制中心,浙江湖州313009

出　　处：《中国食品卫生杂志》2023年第5期768-771,共4页Chinese Journal of Food Hygiene

摘　　要：目的对2021年湖州市南浔区一起聚集性腹泻事件分离的副溶血性弧菌进行病原特征分析,为及时采取控制措施提供依据。方法通过询问患者、医院核查等方法搜索到疑似病例59例,对采集的29份厨师、服务员和病例的肛拭子标本以及18份留存餐食进行病原学检测,采用血清学鉴定、脉冲场凝胶电泳(PFGE)、多重荧光聚合酶链式反应和微量肉汤稀释法分别对副溶血性弧菌分离株进行血清分型、分子分型、tlh/tdh/trh毒力基因检测和药敏试验。结果从3份厨师和6例病例的肛拭子标本中分离到副溶血性弧菌,检出率为15.25%;分离株血清型均为O10:K4。所有菌株tlh、tdh基因均为阳性,trh基因均为阴性。9株分离株仅对氨苄西林耐药,对其他12种抗生素均敏感。经PFGE聚类分析,菌株间相似系数为100.0%,厨师分离株和病例分离株带型一致。结论这是一起由O10:K4副溶血性弧菌引起的聚集性腹泻事件,分离株均携带tdh毒力基因,分离株之间的同源性高。Objective To provide evidence for timely control measures,the pathogenic characteristics of Vibrio parahemolyticus isolated from a cluster diarrhea event in Nanxun District,Huzhou City,in 2021 were analyzed.Methods Fifty-nine suspected cases were identified by interviewing patients and checking hospitals.Twenty-nine anal swab samples were tested for etiology from cooks,waiters,and patients,and 18 food leftovers.Serotyping,molecular typing,tlh/tdh/trh virulence gene detection,and drug sensitivity testing of Vibrio parahaemolyticus isolates were performed by serological identification,pulsed field gel electrophoresis(PFGE),multiplex fluorescent polymerase chain reaction,and micro-broth dilution methods,respectively.Results Vibrio parahaemolyticus was isolated from anal swab samples of 3 cooks and 6 cases,and the detection rate was 15.79%.The serotype of the isolates was O10:K4.All strains were positive for tlh and tdh and negative for trh.Nine isolates were only resistant to ampicillin and sensitive to the other 12 tested antibiotics.PFGE clustering analysis revealed a 100%similarity coefficient between isolates.The band type of isolates from cooks and patients was consistent.Conclusion The aggregation diarrhea was caused by Vibrio parahaemolyticus O10:K4.All isolates harbored the tdh virulence gene.Homology among the isolates was high.

关 键 词：副溶血性弧菌 聚集性腹泻事件 毒力基因 抗生素耐药 血清型O10:K4 

分 类 号：R155[医药卫生—营养与食品卫生学]