负载丁香酚的改性纳米粒对玉米醇溶蛋白膜性能的影响  

Effects of Modified Nanoparticles Loaded with Eugenol on Properties of Zein Films

作　　者：刘锦渊 刘晓丽[1] 夏文水[1] LIU Jinyuan;LIU Xiaoli;XIA Wenshui(School of Food Science and Technology/State Key Laboratory of Food Science and Technology/Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品学院/食品科学与技术国家重点实验室/江苏省食品安全与质量控制协同创新中心,江苏无锡214122

出　　处：《食品科学技术学报》2023年第5期123-135,共13页Journal of Food Science and Technology

基　　金：国家大宗淡水鱼产业技术体系项目(CARS-45-28)。

摘　　要：为开发具有保鲜效果且可生物降解的复合膜,将咖啡酸(CA)通过碳二亚胺介导的偶联反应与氨基-介孔二氧化硅纳米粒(NH2-MSN)共价接枝后负载丁香酚(EG),制备得到丁香酚/咖啡酸-介孔二氧化硅纳米粒(EG/CA-MSN)。以玉米醇溶蛋白(Zein)为成膜基质,EG/CA-MSN为功能性填料,通过流延法进一步得到丁香酚/咖啡酸-介孔二氧化硅纳米粒/玉米醇溶蛋白(EG/CA-MSN/Zein)复合膜。研究了EG/CA-MSN添加量对膜理化及功能特性的影响。扫描电子显微镜和红外光谱分析结果表明,EG/CA-MSN与Zein膜基质之间具有良好的生物相容性,且存在分子间相互作用。EG/CA-MSN的加入可以改善膜的阻隔性能和机械性能,当EG/CA-MSN添加量为9%时,复合膜水蒸气透过率最低[3.11×10^(-9)g·mm/(m^(2)·s·Pa)],拉伸强度可达到18.86 MPa,明显优于单一Zein膜。当EG/CA-MSN添加量为12%时,复合膜表现出良好的抗氧化和抑菌性能,DPPH和ABTS+自由基清除率分别提高到78.28%和85.49%,对大肠杆菌和金黄色葡萄球菌抑菌圈直径分别可达13.57 mm和12.38 mm。将复合膜应用于冷藏鱼片的保鲜,可以有效延缓冷藏鱼片在贮藏期间挥发性盐基氮的积累以及脂肪氧化。研究结果表明,研制的EG/CA-MSN/Zein复合膜在水产保鲜方面具有潜在的应用价值。To develop biodegradable composite films with fresh-keeping effects,caffeic acid(CA)was covalently grafted with amino-mesoporous silica nanoparticles(NH 2-MSN)via a carbodiimide mediated coupling reaction and loaded with eugenol(EG)to obtain eugenol/caffeic acid-mesoporous silica nanoparticles(EG/CA-MSN).Zein was used as film-forming matrix and EG/CA-MSN used as functional filler.Eugenol/caffeic acid-mesoporous silica nanoparticles/Zein(EG/CA-MSN/Zein)composite films were further obtained by flow casting method.The effects of the added amount of EG/CA-MSN on the physicochemical and functional properties of the composite films were investigated.SEM and FTIR results showed that EG/CA-MSN demonstrated good biocompatibility and intermolecular interactions with zein film matrix.The addition of EG/CA-MSN could improve the barrier properties and mechanical properties of the composite films.When the mass fraction of EG/CA-MSN was 9%,and the water vapor transmission rate of composite films was the lowest[3.11×10^(-9) g·mm/(m^(2)·s·Pa)],the tensile strength could reach 18.86 MPa,which was significantly better than the single zein film.When the mass fraction of EG/CA-MSN was 12%,the composite films demonstrated decent antioxidant and bacteriostatic properties.The free radical scavenging rate of DPPH and ABTS+increased to 78.28% and 85.49%,respectively.The diameter of inhibition zone against Escherichia coli and Staphyloccus aureus reached 13.57 mm and 12.38 mm,respectively.The application of the composite films in the preservation of frozen fillets could effectively delay the total volatile basic nitrogen and fat oxidation during storage.The developed EG/CA-MSN/Zein composite film had potential applications value in the preservation of aquatic products.

关 键 词：咖啡酸 丁香酚 介孔二氧化硅 玉米醇溶蛋白 复合膜 保鲜 

分 类 号：TS201.2[轻工技术与工程—食品科学] TS206.4[轻工技术与工程—食品科学与工程]