天仙藤全长转录组测序及分析  

Sequencing and Analysis of Full-length Transcriptome of Fibraurea recisa Pierre

作　　者：黄素华 韦范[1] 全昌乾 唐美琼[1] 胡营[1] 韦筱媚[1] 朱艳霞[1] 韦坤华[1] 黄媛 汤丹峰 缪剑华[1] HUANG Su-hua;WEI Fan;QUAN Chang-qian;TANG Mei-qiong;HU Ying;WEI Xiao-mei;ZHU Yan-xia;WEI Kun-hua;HUANG Yuan;TANG Dan-feng;MIAO Jian-hua(Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/Guangxi Engineering Research Center of TCM Resource Intelligent Creation,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China;School of Pharmacy,Guangxi Medical University,Nanning 530023,China)

机构地区：[1]广西壮族自治区药用植物园、广西药用资源保护与遗传改良重点实验室/广西壮族自治区中药资源智慧创制工程研究中心,广西南宁530023 [2]广西医科大学药学院,广西南宁530023

出　　处：《中国现代中药》2023年第7期1473-1485,共13页Modern Chinese Medicine

基　　金：国家自然科学基金项目(82260750);广西创新驱动发展专项(桂科AA18242040);广西科技计划项目(桂科AD22080012);对发展中国家科技援助项目(KY201904001);中央引导地方科技发展资金项目(桂科ZY22096020)。

摘　　要：目的:获得天仙藤全长转录组数据库,挖掘天仙藤功能基因。方法:采用PacBio SequeⅠ高通量测序系统,对天仙藤的茎、叶、果3个部位的混合样品进行全长转录组测序及分析。结果:共获得176354条环形一致性序列(CCS),获得184439个高质量isoforms,并注释了139826个isoforms,检测出3058个转录因子和135527个简单序列重复(SSR)位点,还预测到76862个长链非编码RNA(lncRNA)和36204个mRNAs。结论:获得了较可靠的天仙藤全长转录组数据,可为深入研究天仙藤基因组、生物学特性、相关代谢途径、信号通路及其分子机制提供数据支持。Objective:To obtain the full-length transcriptome database of Fibraurea recisa Pierre and explore its functional genes.Methods:The mixed samples of stems,leaves,and fruits of F.recisa were subjected to full-length transcriptome sequencing and analysis using the PacBio SequelⅠhigh-throughput sequencing system.Results:A total of 176354 CCS sequences were obtained,resulting in 184439 high-quality isoforms.Among them,139826 isoforms were annotated,and 3058 transcription factors and 135527 SSR sites were detected.Additionally,76862 lncRNAs and 36204 mRNAs were predicted.Conclusion:A reliable full-length transcriptome dataset of F.recisa was obtained,providing valuable data support for in-depth research on the genome,biological characteristics,relevant metabolic pathways,signaling pathways,and molecular mechanisms of F.recisa.

关 键 词：天仙藤 全长转录组 测序分析 功能注释 

分 类 号：R282.5[医药卫生—中药学]