氨基硅烷功能化磁性材料固定化琼胶酶的性能  

Agarase immobilization performance of aminosilane-functionalized magnetic materials

作　　者：王露洁 莫创荣 陆祖逸 邓冬祝 廖丹伶 许雪棠[2] WANG Lujie;MO Chuangrong;LU Zuyi;DENG Dongzhu;LIAO Danling;XU Xuetang(School of Resources,Environment and Materials,Guangxi University,Nanning 530000,Guangxi,China;School of Chemistry and Chemical Engineering,Guangxi University,Nanning 530000,Guangxi,China)

机构地区：[1]广西大学资源环境与材料学院,广西南宁530000 [2]广西大学化学化工学院,广西南宁530000

出　　处：《精细化工》2023年第8期1720-1726,共7页Fine Chemicals

基　　金：国家自然科学基金项目(22065003)。

摘　　要：利用3-氨丙基三乙氧基硅烷(APTES)对制备的SiO2包覆Fe_(3)O_(4)复合粒子(Fe_(3)O_(4)@SiO2)进行改性,制备了氨基硅烷功能化磁性材料Fe_(3)O_(4)@SiO2-NH2,将Fe_(3)O_(4)@SiO2-NH2作为载体用于固定化琼胶酶。采用SEM、FTIR、VSM对Fe_(3)O_(4)@SiO2-NH2和固定化琼胶酶进行了表征,对琼胶酶的固定化条件进行了优化,评价了固定化琼胶酶的性能。结果表明,琼胶酶成功固定在载体上。Fe_(3)O_(4)@SiO2-NH2的磁化饱和强度为48.4 emu/g,固定化琼胶酶的磁化饱和强度为42.8 emu/g,二者均具有较高的磁性。在加酶量为7 m L、戊二醛加入量为4 m L、交联时间2 h、固定时间2 h条件下,酶活回收率为67.74%。与游离琼胶酶相比,固定化琼胶酶具有更强的热稳定性和pH稳定性,其在使用7次后相对活性为40.41%。Aminosilane-functionalized magnetic materials(Fe_(3)O_(4)@SiO2-NH2)was synthesized from SiO2-coated Fe_(3)O_(4)composite particles(Fe_(3)O_(4)@SiO2)and 3-aminopropyltriethoxysilane(APTES),and then used for agarose immobilization.The Fe_(3)O_(4)@SiO2-NH2 and immobilized agarase were characterized by SEM,FTIR and VSM,followed by optimization on the agarase immobilization conditions and evaluation on the the properties of the immobilized agarase.The results showed that agarase was successfully immobilized on the Fe_(3)O_(4)@SiO2-NH2.The magnetic saturation intensity of Fe_(3)O_(4)@SiO2-NH2 was 48.4 emu/g,while that of immobilized agarase was 42.8 emu/g,both of which shoed high magnetic properties.The enzyme activity recovery was 67.74%under the conditions of enzyme dosage 7 mL,glutaraldehyde dosage 4 mL,cross-linking time 2 h,and immobilization time 2 h.The immobilized agarase displayed stronger thermal stability and pH stability than free agarase.Moreover,the relative activity of the immobilized agarase was maintained at 40.41%after 7 recycles.

关 键 词：琼胶酶 固定化 磁性材料 氨基硅烷 琼胶寡糖 功能材料 

分 类 号：Q814[生物学—生物工程]