三七悬浮细胞生物合成绿原酸类物质及其生物活性  

Biosynthesis and Bioactivity of Chlorogenic Acids in Panax notoginseng Suspension Cells

作　　者：周倍 刘楠 刘泽波 庄英萍[1] 王泽建[1] 郭美锦[1] ZHOU Bei;LIU Nan;LIU Zebo;ZHUANG Yingping;WANG Zejian;GUO Meijin(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《华东理工大学学报（自然科学版）》2023年第4期515-528,共14页Journal of East China University of Science and Technology

基　　金：国家重点研发计划(2020YFA0906800)。

摘　　要：利用三七(Panax notoginseng)悬浮细胞生物合成绿原酸类物质(CGAs),并对其生物活性进行了研究。对三七细胞中CGAs进行定性分析,优化了细胞培养条件以及6种诱导模式,测定了三七细胞CGAs的抗氧化及α-葡萄糖苷酶活性抑制能力。结果表明三七愈伤组织内含有4种CGAs;最优工艺条件为:B5+40 g/L蔗糖,pH 7.5,15%接种量;经30μmol/L茉莉酸甲酯诱导3 d后,CGAs产量(684.07 mg/L)比原工艺(475.18 mg/L)提高了0.44倍;三七细胞CGAs具有良好的抗氧化及α-葡萄糖苷酶活性抑制能力。In this paper,Panax notoginseng(P.notoginseng)suspension cells were used for the production of chlorogenic acids(CGAs),of which bioactivities were evaluated.Firstly,CGAs in P.notoginseng suspension cells were identified by liquid chromatography-tandem mass spectrometry.Secondly,the suspension culture system and elicitation mode were optimized.Ultimately,the antioxidant andα-glucosidase inhibitory activity of CGAs from P.notoginseng cells were determined.Four CGAs were inferred in P.notoginseng cells.The optimal condition was obtained(B5+40 g/L sucrose,pH 7.5 and 15%inoculation size).By co-culture with 30μmol/L methyl jasmonate for 3 d,the CGAs yield could reach up to 684.07 mg/L,which was 1.44 times that of the control.CGAs from P.notoginseng cells showed an excellent antioxidant capacity in radical-scavenging test and,moreover,certain inhibition onα-glucosidase activity.Our results indicate that the production of CGAs by P.notoginseng cells has potential applications in healthy food and daily cosmetics.

关 键 词：三七愈伤组织细胞 悬浮培养 绿原酸生物合成 工艺优化 生物活性 

分 类 号：TS201.3[轻工技术与工程—食品科学]