Purification and Characterization of Hyaluronate Lyases Produced by Two Types of Bacteria  

作　　者：Shuai LI Xinhui WANG Yota TATARA Shigeki HAMADA Takuya KOZEKI Kaoru KOJIMA Takashi YOSHIDA 

机构地区：[1]Department of Biochemistry and Molecular Biology,Faculty of Agriculture and Life Science,Hirosaki University,Aomori 036-8561,Japan [2]Department of Stress Response Science,Center for Advanced Medical Research,Hirosaki University Graduate School of Medicine,Aomori 036-8562,Japan [3]Faculty of Agriculture,Yamagata University,Yamagata 997-8555,Japan

出　　处：《Agricultural Biotechnology》2023年第4期99-104,共6页农业生物技术（英文版）

摘　　要：Hyaluronate lyases were obtained from two types of naturally isolated bacterial strains Paenibacillus yunnanensis and Paennarthrobacter nicotinovorans.PyHL(form P.yunnanensis)in the culture supernatant of the bacteria was purified by two steps of column chromatography.The enzyme showed the molecular mass of 74 kDa by SDS-PAGE and the maximal activity at pH 5.0,35℃.PyHL maximally degraded hyaluronate by an endo-type manner,and showed low degradation activity toward chondroitin sulfates.Dermatan sulfate was not the substrate.PnHL(from P.nicotinovorans)in the culture supernatant of the bacteria was purified by two steps of column chromatography.The enzyme showed the molecular mass of 70 kDa by SDS-PAGE and the maximal activity at pH 6.0,30℃.Genomic analysis of P.nicotinovorans on the bases of the internal amino acid sequences of PnHL.

关 键 词：GLYCOSAMINOGLYCAN HYALURONAN Hyaluronate lyase Paenibacillus yunnanensis Paennarthrobacter nicotinovorans 

分 类 号：X173[环境科学与工程—环境科学] X714