响应面法优化方格星虫多肽-钙螯合物的制备工艺  

Preparation of Sipunculus nudus peptide-calcium chelate by response surface method

作　　者：李康 戚怡 蔡程锋 简玮 梁靖岚 蒋雨薇 陈锦灿 罗辉 欧阳茜茜 LI Kang;QI Yi;CAI Cheng-feng;JIAN Wei;LIANG Jing-lan;JIANG Yu-wei;CHEN Jin-can;LUO Hui;OUYANG Qian-qian(Marine Biomedical Research Institution,Guangdong Medical University,Zhanjiang 524023,China;Zhanjiang Marine Biomedical Research Institute of Guangdong province,Zhanjiang 524000,China)

机构地区：[1]广东医科大学海洋医药研究院,广东湛江524023 [2]广东湛江海洋医药研究院,广东湛江524000

出　　处：《广东医科大学学报》2023年第4期383-387,394,共6页Journal of Guangdong Medical University

基　　金：广东医科大学博士科研启动基金(4SG21243G);广东省中医药局科研项目(20232095);广东省水产品加工与安全重点实验室开放基金课题(GDPKLAPPS2206);广东省药品监督管理局科技创新项目(2023TDZ12)。

摘　　要：目的探究方格星虫多肽-钙螯合物的最佳制备工艺。方法通过单因素试验探究螯合反应中肽钙质量比及反应时间、温度、pH对螯合率的影响。通过Box-Behnken响应面试验探究螯合反应的最佳工艺。结果经过Box-Behnken响应面试验的优选,方格星虫多肽-钙螯合物的最佳制备工艺条件为:肽钙质量比5∶1、反应时间30 min、反应温度60℃、反应pH 7.0,经验证试验后方格星虫肽钙螯合率可达68.32%。结论响应面法优化的方格星虫多肽-钙螯合物的制备工艺简单、高效。Objective To study the optimal preparation technique for Sipunculus nudus peptide-calcium chelate(Sn-PCC).Methods The effects of peptide-calcium mass ratio and reaction time,temperature and pH on chelation rate were assessed by single factor experiments.The optimal process for chelation reaction was evaluated by Box-Behnken response surface method.Results After the optimization of Box-Behnken response surface method,the optimal preparation parameters for Sn-PCC were 5∶1 of peptide-calcium mass ratio,30 min,60°C,and pH 7.0.The calcium chelation rate of Sn peptide was 68.32%using verification test.Conclusion The preparation process of Sn-PCC optimized by response surface method is simple and efficient.

关 键 词：方格星虫 多肽 螯合钙 响应面法 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]