维生素B12对高脂饮食大鼠血脂、肝脂及免疫球蛋白的影响  

Effects of vitamin B 12 on blood lipid,liver lipid and immunoglobulin in rats with high-fat diet

作　　者：张大龙[1] 周殿明[1] 张静[1] 钱智勇[1] ZHANG Dalong;ZHOU Dianming;ZHANG Jing;QIAN Zhiyong(Department of Toxicology,Tianjin Centers for Disease Control and Prevention,Tianjin,300011,China)

机构地区：[1]天津市疾病预防控制中心毒理室,天津300011

出　　处：《职业与健康》2023年第15期2054-2058,共5页Occupation and Health

摘　　要：目的 探讨维生素B12对高脂饮食大鼠血脂、肝脂及免疫球蛋白影响。方法 采用高脂饲料喂饲28 d后,将30只雄性(sprauge-dawley,SD)大鼠按血清总胆固醇(total cholesterol,TC)水平随机平均分为3组,维生素B12高剂量组,剂量为0.06mg/kg、维生素B12低剂量组剂量为0.04 mg/kg和高脂饮食组,并继续喂饲高脂饲料。另设10只大鼠为空白对照组,实验期间喂饲基础饲料饮食。干预期为40 d,灌胃方式进行干预,高脂饮食组与空白对照组给予相同体积的生理盐水。干预第0,20,40天采集动物眼内眦静脉血,测定TC、三酰甘油(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)和高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)水平,并记录动物体质量。实验末期,收集各组肝组织,测定肝组织匀浆TC、TG、LDL-C和HDL-C水平,采用ELISA法测定免疫球蛋白M(immunoglobulin M,IgM)、IgG和IgA水平。结果 与空白对照组比较,干预第0天时高脂饮食组及维生素B12各干预组TC、TG和LDL-C水平升高,差异均有统计学意义(均P<0.05);干预第20天时,高脂饮食组TC、TG及LDL-C水平高于空白对照组,差异均有统计学意义(均P<0.05),维生素B12低、高剂量组TC及LDL-C水平高于空白对照组,差异均有统计学意义(均P<0.05);干预第40天时,高脂饮食组TC和LDL-C水平高于空白对照组,差异均有统计学意义(均P<0.05)。与高脂饮食组比较,干预第20天,维生素B12低、高剂量组TC、TG及LDL-C水平降低,差异均有统计学意义(均P<0.05),干预第40天时维生素B12高剂量组TC及维生素B12各剂量组LDL-C水平显著低于高脂饮食组,差异均有统计学意义(均P<0.05)。干预第40天后,高脂饮食组肝脏TC、TG、LDL-C水平显著高于空白对照组(均P<0.05),IgM和IgG水平显著低于空白对照组(均P<0.05);相比于高脂饮食组,叶酸低、高剂量组的肝脏TC和LDL-C水平以及高剂量组TG水平明显降低(F=20.6Objective To explore the effects of vitamin B 12 on blood lipid,liver lipid and immunoglobulin in rats with high-fat diet.Methods After 28 days of feeding high fat feed,30 sprauge-dawley(SD)male rats were divided into three groups equally and randomly by total cholesterol(TC),including high-dose vitamin B 12 group(vitamin B 12 dose was 0.06 mg/kg),low-dose vitamin B 12 group(vitamin B 12 dose was 0.04 mg/kg)and high-fat diet group,and the animals were fed by high fat diets continuously.Another 10 SD rats were given conventional diet as the control group.All rats were oral gavaged daily for 40 days.The high-fat diet group and the control group were given an equivalent amount of normal saline.The intracanthal venous blood samples of the animals were collected at the 0,20th and 40th day after intervention,to measure the levels of TC,triacylglycerol(TG),low density lipoprotein cholesterol(LDL-C)and high density lipoprotein cholesterol(HDL-C),and the body weight of the animals were recorded.At the end of experiment,the liver tissue was collected for measuring the levels of TC,TG,LDL-C,HDL-C in liver tissue homogenate.The ELISA method was used to determine the levels of immunoglobulin M(IgM),IgG and IgA.Results At 0 day of intervention,the levels of serum TC,TG and LDL-C in high-fat diet group and vitamin B 12 intervention groups were higher than those in the control group,and the differences were statistically significant(all P<0.05).At 20th day of intervention,the levels of serum TC,TG and LDL-C in high-fat diet group were higher than those in the control group,and the differences were statistically significant(all P<0.05),while the levels of serum TC and LDL-C in low-dose and high-dose vitamin B 12 groups were higher than those in the control group,and the differences were statistically significant(all P<0.05).At 40th day of intervention,the levels of serum TC and LDL-C in high-fat diet group were higher than those in the control group,and the differences were statistically significant(all P<0.05).At 20th day of int

关 键 词：维生素B12 高脂饮食 血脂 肝脂 免疫球蛋白 

分 类 号：R-332[医药卫生]