m6A去甲基化酶FTO通过调控p53磷酸化抑制急性髓系白血病的发生发展  

m6A Demethylase FTO Inhibiting the Development and Progression of Acute Myeloid Leukemia by Regulating the Phosphorylation of p53

作　　者：华春兰 李海军[1] 喻静 HUA Chunlan;LI Haijun;YU Jing(Key Clinical Laboratory of Henan Province,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院河南省检验医学重点实验室,河南郑州450052

出　　处：《河南医学研究》2023年第16期2912-2916,共5页Henan Medical Research

基　　金：河南省青年科学基金(212300410263)。

摘　　要：目的研究FTO/p53在急性髓系白血病(AML)发生发展中的作用。方法对2018年1月至2019年1月郑州大学第一附属医院收治的27例AML患者和15例健康对照外周血中FTO表达水平进行分析,并结合TCGA数据库分析,深入研究FTO在AML发生发展中的作用。结果在AML患者外周血中,FTO表达水平升高。分析TCGA数据库发现FTO与p53磷酸化存在相关性,使用siRNA敲降白血病细胞系HL60和K562中FTO后,HL60和K562细胞凋亡增多,结果显示FTO表达水平降低后白血病细胞的凋亡增加。进一步通过体外功能实验显示FTO表达降低后p53磷酸化平均荧光强度增强。结论m6A去甲基化酶FTO通过调控p53磷酸化抑制AML的发生发展。Objective To research the role of FTO/p53 in the development of acute myeloid leukemia(AML).Methods The FTO expression level in peripheral blood of 27 AML patients and 15 health control refer to the First Affiliated Hospital of Zhengzhou University from January 2018 to January 2019 were tested,TCGA database analyses was performed to further study the role of FTO in the development of AML.Results The expression of FTO was significantly increased in peripheral blood of AML patients compared with health control.The relationship between FTO and p53 was found through TCGA database analysis.The percentage of apoptosis cells was increased after FTO was knocked down by siRNA in HL60 and K562,the results showed that the apoptosis of leukemia cells increased after the expression level of FTO decreased.in vitro experiments demonstrated that the mean fluorescence intensity of p53 enhanced after the level of FTO decreased,this indicated that the phosphorylation of p53 enhanced after FTO was knocked down.Conclusion m6A demethylase FTO inhibites the development and progression of AML by regulating the phosphorylation of p53.

关 键 词：m6A去甲基酶 FTO P53 急性髓系白血病 

分 类 号：R733.71[医药卫生—肿瘤]